Abstract
The electrophoretic mobility of fixed human erythrocytes immunologically labeled with poly(vinylpyridine) or poly(glutaraldehyde) microspheres was reduced by approximately 40%. This observation was utilized in preparative scale electrophoretic separations of fixed human and turkey erythrocytes, the mobilities of which under normal physiological conditions do not differ sufficiently to allow their separation by continuous flow electrophoresis. We suggest that resolution in the electrophoretic separation of cell subpopulations, currently limited by finite and often overlapping mobility distribution, may be significantly enhanced by immunospecific labeling of target populations using microspheres.
| Original language | English |
|---|---|
| Pages (from-to) | 246-255 |
| Number of pages | 10 |
| Journal | Biochimica et Biophysica Acta - General Subjects |
| Volume | 588 |
| Issue number | 2 |
| DOIs | |
| State | Published - 3 Dec 1979 |
| Externally published | Yes |
Bibliographical note
Funding Information:The authors thank the Universities Space Research Association and the National Aeronautics and Space Administration for financial support. We thank also Dr. R.S. Molday for helpful discussions and Ms. J.K. McGuire for skilled assistance. Special thanks are due to Dr. R.S. Snyder, Marshall Space Flight Center, for useful comments and provision of laboratory facilities.
Keywords
- (Electrophoresis)
- Cell separation
- Immunospecific labeling
- Microsphere
- Poly (glutaraldehyde)
- Poly(vinylpyridine)