TY - JOUR
T1 - Eicosapentaenoic acid release from the red alga Pachymeniopsis lanceolata by enzymatic degradation
AU - Matsukawa, Ritsuko
AU - Hatakeda, Kiyotaka
AU - Ito, Shota
AU - Numata, Yukiyo
AU - Nakamachi, Hidetoshi
AU - Hasebe, Yasushi
AU - Uchiyama, Shunichi
AU - Notoya, Masahiro
AU - Dubinsky, Zvy
AU - Karube, Isao
PY - 1999
Y1 - 1999
N2 - Forty-eight species of seaweeds from Japanese waters were screened for the valuable polyunsaturated fatty acids eicosapentaenoic acid (EPA). The eight species that contained the highest levels of these compounds were analyzed in detail. Of all species tested the red alga Pachymeniopsis lanceolata contained the highest EPA concentration, and it was present as both the free and bound forms. EPA constituted 38.7% of total fatty acids, and polar lipids were the main constituent of the total lipids in P.lanceolata. EPA was obtained from the marine algae P.lanceolata by enzymatic hydrolysis of the total lipids extract using phospholipase A2 (PLA2). The release of EPA reached a plateau after 10 min of enzymatic treatment. These results suggest that P.lanceolata is a useful natural source of EPA and that PLA2 treatment is a convenient method for obtaining EPA from the red alga.
AB - Forty-eight species of seaweeds from Japanese waters were screened for the valuable polyunsaturated fatty acids eicosapentaenoic acid (EPA). The eight species that contained the highest levels of these compounds were analyzed in detail. Of all species tested the red alga Pachymeniopsis lanceolata contained the highest EPA concentration, and it was present as both the free and bound forms. EPA constituted 38.7% of total fatty acids, and polar lipids were the main constituent of the total lipids in P.lanceolata. EPA was obtained from the marine algae P.lanceolata by enzymatic hydrolysis of the total lipids extract using phospholipase A2 (PLA2). The release of EPA reached a plateau after 10 min of enzymatic treatment. These results suggest that P.lanceolata is a useful natural source of EPA and that PLA2 treatment is a convenient method for obtaining EPA from the red alga.
KW - Eicosapentaenoic acid
KW - Enzymatic hydrolysis
KW - Pachymeniopsis lanceolata
KW - Phospholipase A
KW - Seaweeds
UR - http://www.scopus.com/inward/record.url?scp=0032774961&partnerID=8YFLogxK
U2 - 10.1385/ABAB:80:2:141
DO - 10.1385/ABAB:80:2:141
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C2 - 10436916
AN - SCOPUS:0032774961
SN - 0273-2289
VL - 80
SP - 141
EP - 150
JO - Applied Biochemistry and Biotechnology
JF - Applied Biochemistry and Biotechnology
IS - 2
ER -
