Efficient procedure and methods to determine critical electroporation parameters

Gaddi Blumrosen; Alireza Abazari; Alexander Golberg; Mehmet Tonner; Martin L. Yarmush

doi:10.1109/cbms.2014.18

Efficient procedure and methods to determine critical electroporation parameters

Gaddi Blumrosen, Alireza Abazari, Alexander Golberg, Mehmet Tonner, Martin L. Yarmush

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

9 Scopus citations

Abstract

Controlling electroporation and determination of its design parameters govern the performance in many electroporation applications in the fields of medicine, biology, and food industry. This work provides an enhanced high throughput signal processing method to enable non-expert, objective, automatic and fast analysis of large datasets of microscopic images of high throughput electroporation experiments. This can enable optimal design of vital electroporation parameters including the optimal thresholds for the electric field strength, and the optimal number of pulses. In this work the non-expert visualization platform and the methods are described, and implemented on normal dermal human fibroblasts cell culture.

Original language	English
Title of host publication	Proceedings - 2014 IEEE 27th International Symposium on Computer-Based Medical Systems, CBMS 2014
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	314-318
Number of pages	5
ISBN (Print)	9781479944354
DOIs	https://doi.org/10.1109/cbms.2014.18
State	Published - 2014
Externally published	Yes
Event	27th IEEE International Symposium on Computer-Based Medical Systems, CBMS 2014 - New York, NY, United States Duration: 27 May 2014 → 29 May 2014

Publication series

Name	Proceedings - IEEE Symposium on Computer-Based Medical Systems
ISSN (Print)	1063-7125

Conference

Conference	27th IEEE International Symposium on Computer-Based Medical Systems, CBMS 2014
Country/Territory	United States
City	New York, NY
Period	27/05/14 → 29/05/14

Keywords

Biological system modeling
Electroporation
Image processing
Light Microscopy
clustering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1109/cbms.2014.18

Cite this

Blumrosen, G., Abazari, A., Golberg, A., Tonner, M., & Yarmush, M. L. (2014). Efficient procedure and methods to determine critical electroporation parameters. In Proceedings - 2014 IEEE 27th International Symposium on Computer-Based Medical Systems, CBMS 2014 (pp. 314-318). Article 6881898 (Proceedings - IEEE Symposium on Computer-Based Medical Systems). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/cbms.2014.18

Blumrosen, Gaddi ; Abazari, Alireza ; Golberg, Alexander et al. / Efficient procedure and methods to determine critical electroporation parameters. Proceedings - 2014 IEEE 27th International Symposium on Computer-Based Medical Systems, CBMS 2014. Institute of Electrical and Electronics Engineers Inc., 2014. pp. 314-318 (Proceedings - IEEE Symposium on Computer-Based Medical Systems).

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keywords = "Biological system modeling, Electroporation, Image processing, Light Microscopy, clustering",

author = "Gaddi Blumrosen and Alireza Abazari and Alexander Golberg and Mehmet Tonner and Yarmush, {Martin L.}",

year = "2014",

doi = "10.1109/cbms.2014.18",

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booktitle = "Proceedings - 2014 IEEE 27th International Symposium on Computer-Based Medical Systems, CBMS 2014",

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Blumrosen, G, Abazari, A, Golberg, A, Tonner, M & Yarmush, ML 2014, Efficient procedure and methods to determine critical electroporation parameters. in Proceedings - 2014 IEEE 27th International Symposium on Computer-Based Medical Systems, CBMS 2014., 6881898, Proceedings - IEEE Symposium on Computer-Based Medical Systems, Institute of Electrical and Electronics Engineers Inc., pp. 314-318, 27th IEEE International Symposium on Computer-Based Medical Systems, CBMS 2014, New York, NY, United States, 27/05/14. https://doi.org/10.1109/cbms.2014.18

Efficient procedure and methods to determine critical electroporation parameters. / Blumrosen, Gaddi; Abazari, Alireza; Golberg, Alexander et al.
Proceedings - 2014 IEEE 27th International Symposium on Computer-Based Medical Systems, CBMS 2014. Institute of Electrical and Electronics Engineers Inc., 2014. p. 314-318 6881898 (Proceedings - IEEE Symposium on Computer-Based Medical Systems).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AB - Controlling electroporation and determination of its design parameters govern the performance in many electroporation applications in the fields of medicine, biology, and food industry. This work provides an enhanced high throughput signal processing method to enable non-expert, objective, automatic and fast analysis of large datasets of microscopic images of high throughput electroporation experiments. This can enable optimal design of vital electroporation parameters including the optimal thresholds for the electric field strength, and the optimal number of pulses. In this work the non-expert visualization platform and the methods are described, and implemented on normal dermal human fibroblasts cell culture.

KW - Biological system modeling

KW - Electroporation

KW - Image processing

KW - Light Microscopy

KW - clustering

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Blumrosen G, Abazari A, Golberg A, Tonner M, Yarmush ML. Efficient procedure and methods to determine critical electroporation parameters. In Proceedings - 2014 IEEE 27th International Symposium on Computer-Based Medical Systems, CBMS 2014. Institute of Electrical and Electronics Engineers Inc. 2014. p. 314-318. 6881898. (Proceedings - IEEE Symposium on Computer-Based Medical Systems). doi: 10.1109/cbms.2014.18

Efficient procedure and methods to determine critical electroporation parameters

Abstract

Publication series

Conference

Keywords

UN SDGs

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