Effects of membrane physical parameters on hematoporphyrin-derivative binding to liposomes: A spectroscopic study

Eitan Gross, Zvi Malik, Benjamin Ehrenberg

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17 Scopus citations

Abstract

Physical parameters of membrane bilayers were studied for their effect on the binding of hematoporphyrin derivative (Hpd), which is used as a sensitizer in photodynamic therapy of cancerous tissues. The purpose of this study was to clarify which parameters were relevant, under physiological conditions, to the selectivity of Hpd binding to cancer cells. Fluorescence spectroscopy was used to measure the relative partitioning of the dye between the lipid and aqueous media. Increasing the microviscosity of the liposomes' membranes by various bilayer additives results in a strong reduction of Hpd binding, to an extent independent of the specific additive. The effect of temperature near the physiological value as well as the effect of cross membrane potential are small. Surface potential does not affect the binding constant, indicating that the binding species does not carry a net electric charge.

Original languageEnglish
Pages (from-to)215-221
Number of pages7
JournalJournal of Membrane Biology
Volume97
Issue number3
DOIs
StatePublished - Oct 1987

Keywords

  • dye-liposome interaction
  • fluorescence polarization
  • hematoporphyrin derivative
  • membrane electric potentials
  • membrane order and microviscosity

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