The effects of incubation of cultured rat skeletal myotubes in ouabain were studied on the number of [3H]ouabain binding sites and electrogenic component of membrane potential. Ouabain treatment for 2-6 days increased the number of binding sites, resting membrane potential (Em) and the ouabain-sensitive component of Em in the muscle cells. The findings strengthen the idea that Na, K-ATPase has an important role in regulation of Em in cultured skeletal muscle and suggest that Na-K pump inhibition during development may be a regulatory mechanism for cellular synthesis of Na, K-ATPase.
|Number of pages||3|
|State||Published - 11 Nov 1985|
Bibliographical noteFunding Information:
study was supported by funds from the Research Authority, Bar-Ilan University and Yad Hanadiv, Tel-Aviv, Israel.
- Na, K-ATPase
- cultured skeletal myotube
- membrane potential
- ouabain binding site