Effects of Antioxidants Apocynin and the Natural Water-Soluble Antioxidant from Spinach on Cellular Damage Induced by Lipopolysaccaride in the Rat

Liat Lomnitski, Abraham Nyska, Varda Ben-Shaul, Robert R. Maronpot, Joseph K. Haseman, Tal Levin Harrus, Margalit Bergman, Shlomo Grossman

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Oxidative damage plays a key role in septic shock induced by the endotoxin lipopolysaccaride (LPS) by enhancing the formation of reactive oxygen species such as superoxide anion radicals, peroxides, and their secondary product, malondialdehyde, especially in the liver. In this study, histopathologic changes in several organs were compared among groups of male Wistar rats that had been injected with LPS following prophylactic pretreatment with either of 2 antioxidants, a group that had been injected with LPS without pretreatment with antioxidants, an untreated control group, and groups that had been injected with either of the 2 antioxidants only. The antioxidants used were a water-soluble natural antioxidant from spinach (NAO) and the NADPH oxidase inhibitor apocynin. Hematoxylin-and-eosin-stained slides were prepared, and lesions were semiquantitatively scored. Exposure to LPS alone was associated with multifocal hepatocellular necrosis and acute inflammation, thymic and splenic lymphoid necrosis, ocular retinal hemorrhage and acute endophthalmitis, adrenal medullary vacuolation and necrosis and acute inflammation, and decreased adrenal cortical cytoplasmic vacuolation (consistent with depletion of steroidal hormone contents). Results indicated that pretreatment with both antioxidants for 8 days reduced, in some organs, the necrotic and inflammatory changes associated with the LPS challenge. These findings suggest a potential therapeutic application for these antioxidants in clinical sepsis.

Original languageEnglish
Pages (from-to)580-587
Number of pages8
JournalToxicologic Pathology
Volume28
Issue number4
DOIs
StatePublished - Jul 2000
Externally publishedYes

Funding

FundersFunder number
National Institute of Environmental Health SciencesZIAES045005

    Keywords

    • Oxidative stress
    • adrenal
    • liver
    • necrosis
    • spleen
    • thymus

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