Abstract
Monte Carlo simulations are used to study the effect of spontaneous (intrinsic) twist on the conformation of topologically equilibrated minicircles of dsDNA. The twist, writhe, and radius of gyration distributions and their moments are calculated for different spontaneous twist angles and DNA lengths. The average writhe and twist deviate in an oscillatory fashion (with the period of the double helix) from their spontaneous values, as one spans the range between two neighboring integer values of intrinsic twist. Such deviations vanish in the limit of long DNA plasmids.
| Original language | English |
|---|---|
| Pages (from-to) | 2987-2994 |
| Number of pages | 8 |
| Journal | Biophysical Journal |
| Volume | 99 |
| Issue number | 9 |
| DOIs | |
| State | Published - 3 Nov 2010 |
Bibliographical note
Funding Information:This work was supported by grants from the Israel Science Foundation and the Israel-France Research Networks Program in Biophysics and Physical Biology, and by the Materials Research Science and Engineering Center program of the National Science Foundation No. DMR-0520513 at Northwestern University.
Funding
This work was supported by grants from the Israel Science Foundation and the Israel-France Research Networks Program in Biophysics and Physical Biology, and by the Materials Research Science and Engineering Center program of the National Science Foundation No. DMR-0520513 at Northwestern University.
| Funders | Funder number |
|---|---|
| Israel-France Research Networks Program in Biophysics and Physical Biology | |
| National Science Foundation | |
| Directorate for Mathematical and Physical Sciences | 0520513 |
| Materials Research Science and Engineering Center, Harvard University | |
| Israel Science Foundation |