Effect of Leiurus quinquestriatus hebreus venom on calcium and deoxyglucose uptake in cultured cardiac cells

Galit Arie-Saadia, Shaul Sofer, Eli Zlotkin, Asher Shainberg

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    9 Scopus citations

    Abstract

    The effects of scorpion venom Leiurus quinquestriatus hebreus were studied on cardiac cells grown in culture. The venom (30 μg/ml) increased significantly (P < 0.05) Ca2+ uptake into intact cardiocytes and to sarcoplasmic reticulum of skinned cells. [3H]Deoxyglucose uptake was also increased significantly (P < 0.05) in venom treated cardiocytes. It was found that fractions I and III of the venom, separated by gel filtration and ion exchange chromatography, are responsible for the increased Ca2+ uptake by the sarcoplasmic reticulum, whereas fraction IIb, III and IV are responsible for the accelerated rate of uptake of 45Ca and [3H]deoxyglucose by intact cells. Ca channel blockers prevented these effects and similar results were obtained by propranolol. Thus, it is concluded that the venom exerts its effect through activation of β-adrenoceptors which causes the opening of L-type Ca channels.

    Original languageEnglish
    Pages (from-to)435-442
    Number of pages8
    JournalToxicon
    Volume34
    Issue number4
    DOIs
    StatePublished - Apr 1996

    Bibliographical note

    Funding Information:
    Acknowledgements-We are indebted to MS T. Zinman and MS A. Isaac for their valuable technical assistance. This work was partially supported by funds from the Otto Meyerhoff Drug Receptor Center at Bar-Ilan University.

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