Effect of kinetin and GA3 on in vitro ovule embryo culture of Cucumis melo L.

Alexander Beharav; Yigal Cohen

doi:10.1007/bf00024785

Effect of kinetin and GA₃ on in vitro ovule embryo culture of Cucumis melo L.

Alexander Beharav, Yigal Cohen

Bar-Ilan University

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

The ability of Cucumis melo embryos of different ages to form plants in vitro was studied in order to rescue hybrid embryos between C. melo and Cucumis metuliferus. Plants were grown in a glasshouse at temperatures ranging from 15°CN-28°CD. Best results were obtained with ovule embryos excised 17 days after pollination. At this age, kinetin of 0.5 mg l^-1 was found optimal for culturing embryo development. Similar results were obtained with ovule embryos excised 14 days after pollination which cultured on 0.5 mg l^-1 kinetin with 0.5 mg l^-1 GA₃.

Original language	English
Pages (from-to)	267-269
Number of pages	3
Journal	Plant Growth Regulation
Volume	16
Issue number	3
DOIs	https://doi.org/10.1007/bf00024785
State	Published - May 1995

Keywords

Cucumis melo
GA
embryo rescue
immature embryos
kinetin
precocious germination

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title = "Effect of kinetin and GA3 on in vitro ovule embryo culture of Cucumis melo L.",

abstract = "The ability of Cucumis melo embryos of different ages to form plants in vitro was studied in order to rescue hybrid embryos between C. melo and Cucumis metuliferus. Plants were grown in a glasshouse at temperatures ranging from 15°CN-28°CD. Best results were obtained with ovule embryos excised 17 days after pollination. At this age, kinetin of 0.5 mg l-1 was found optimal for culturing embryo development. Similar results were obtained with ovule embryos excised 14 days after pollination which cultured on 0.5 mg l-1 kinetin with 0.5 mg l-1 GA3.",

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author = "Alexander Beharav and Yigal Cohen",

year = "1995",

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AU - Beharav, Alexander

AU - Cohen, Yigal

PY - 1995/5

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N2 - The ability of Cucumis melo embryos of different ages to form plants in vitro was studied in order to rescue hybrid embryos between C. melo and Cucumis metuliferus. Plants were grown in a glasshouse at temperatures ranging from 15°CN-28°CD. Best results were obtained with ovule embryos excised 17 days after pollination. At this age, kinetin of 0.5 mg l-1 was found optimal for culturing embryo development. Similar results were obtained with ovule embryos excised 14 days after pollination which cultured on 0.5 mg l-1 kinetin with 0.5 mg l-1 GA3.

AB - The ability of Cucumis melo embryos of different ages to form plants in vitro was studied in order to rescue hybrid embryos between C. melo and Cucumis metuliferus. Plants were grown in a glasshouse at temperatures ranging from 15°CN-28°CD. Best results were obtained with ovule embryos excised 17 days after pollination. At this age, kinetin of 0.5 mg l-1 was found optimal for culturing embryo development. Similar results were obtained with ovule embryos excised 14 days after pollination which cultured on 0.5 mg l-1 kinetin with 0.5 mg l-1 GA3.

KW - Cucumis melo

KW - GA

KW - embryo rescue

KW - immature embryos

KW - kinetin

KW - precocious germination

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ER -

Effect of kinetin and GA₃ on in vitro ovule embryo culture of Cucumis melo L.

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