Effect of endogenous expression of the laminin α5 chain on chemotherapy resistance of colorectal cancer cells

D. V. Maltseva; M. P. Raigorodskaya; G. A. Belyakova; A. A. Turchinovich

doi:10.21519/0234-2758-2019-35-5-29-35

Effect of endogenous expression of the laminin α5 chain on chemotherapy resistance of colorectal cancer cells

D. V. Maltseva
, M. P. Raigorodskaya
, G. A. Belyakova
, A. A. Turchinovich

Russian Academy of Sciences
BioClinicum Research Center
Sechenov First Moscow State Medical University
SciBerg e.Kfm
German Cancer Research Center

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The expression of the LAMA5 gene encoding the laminin a5 chain has been inhibited in the line HT29 cells. The treatment with 5-fluorouracil (5FU) and regorafenib (RF) showed that the knockdown of LAMA5 increases the sensitivity of the HT29 cells to 5FU by 2.2 times and does not affect their sensitivity to RF. The data of transcriptome analysis indicate that the observed changes in the sensitivity to 5FU may be caused by an activation of signaling pathways of the endoplasmic reticulum stress in the HT29 cells. In the case of RF, this effect can be compensated by a 2-fold decrease in the expression of the main RF transporter in HT29 cells.

Original language	English
Pages (from-to)	29-35
Number of pages	7
Journal	Biotekhnologiya
Volume	35
Issue number	5
DOIs	https://doi.org/10.21519/0234-2758-2019-35-5-29-35
State	Published - 2019
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2019 State Research Institute for Genetics and Selection of Industrial Microorganisms.

Funding

The study was supported by the Russian Science Foundation (Project 17-14-01338).

Funders	Funder number
Russian Science Foundation	17-14-01338

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

5-fluorouracil
Apoptosis
Colorectal cancer
Drug resistance
Endogenous expression
Endoplasmic reticulum stress
HT29
Knockdown
LAMA5
Laminin a5 chain
Laminins
Regorafenib
UPR

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10.21519/0234-2758-2019-35-5-29-35

Cite this

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title = "Effect of endogenous expression of the laminin α5 chain on chemotherapy resistance of colorectal cancer cells",

abstract = "The expression of the LAMA5 gene encoding the laminin a5 chain has been inhibited in the line HT29 cells. The treatment with 5-fluorouracil (5FU) and regorafenib (RF) showed that the knockdown of LAMA5 increases the sensitivity of the HT29 cells to 5FU by 2.2 times and does not affect their sensitivity to RF. The data of transcriptome analysis indicate that the observed changes in the sensitivity to 5FU may be caused by an activation of signaling pathways of the endoplasmic reticulum stress in the HT29 cells. In the case of RF, this effect can be compensated by a 2-fold decrease in the expression of the main RF transporter in HT29 cells.",

keywords = "5-fluorouracil, Apoptosis, Colorectal cancer, Drug resistance, Endogenous expression, Endoplasmic reticulum stress, HT29, Knockdown, LAMA5, Laminin a5 chain, Laminins, Regorafenib, UPR",

author = "Maltseva, \{D. V.\} and Raigorodskaya, \{M. P.\} and Belyakova, \{G. A.\} and Turchinovich, \{A. A.\}",

note = "Publisher Copyright: {\textcopyright} 2019 State Research Institute for Genetics and Selection of Industrial Microorganisms.",

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doi = "10.21519/0234-2758-2019-35-5-29-35",

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T1 - Effect of endogenous expression of the laminin α5 chain on chemotherapy resistance of colorectal cancer cells

AU - Maltseva, D. V.

AU - Raigorodskaya, M. P.

AU - Belyakova, G. A.

AU - Turchinovich, A. A.

PY - 2019

Y1 - 2019

N2 - The expression of the LAMA5 gene encoding the laminin a5 chain has been inhibited in the line HT29 cells. The treatment with 5-fluorouracil (5FU) and regorafenib (RF) showed that the knockdown of LAMA5 increases the sensitivity of the HT29 cells to 5FU by 2.2 times and does not affect their sensitivity to RF. The data of transcriptome analysis indicate that the observed changes in the sensitivity to 5FU may be caused by an activation of signaling pathways of the endoplasmic reticulum stress in the HT29 cells. In the case of RF, this effect can be compensated by a 2-fold decrease in the expression of the main RF transporter in HT29 cells.

AB - The expression of the LAMA5 gene encoding the laminin a5 chain has been inhibited in the line HT29 cells. The treatment with 5-fluorouracil (5FU) and regorafenib (RF) showed that the knockdown of LAMA5 increases the sensitivity of the HT29 cells to 5FU by 2.2 times and does not affect their sensitivity to RF. The data of transcriptome analysis indicate that the observed changes in the sensitivity to 5FU may be caused by an activation of signaling pathways of the endoplasmic reticulum stress in the HT29 cells. In the case of RF, this effect can be compensated by a 2-fold decrease in the expression of the main RF transporter in HT29 cells.

KW - 5-fluorouracil

KW - Apoptosis

KW - Colorectal cancer

KW - Drug resistance

KW - Endogenous expression

KW - Endoplasmic reticulum stress

KW - HT29

KW - Knockdown

KW - LAMA5

KW - Laminin a5 chain

KW - Laminins

KW - Regorafenib

KW - UPR

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JF - Biotekhnologiya

IS - 5

ER -

Effect of endogenous expression of the laminin α5 chain on chemotherapy resistance of colorectal cancer cells

Abstract

Bibliographical note

Funding

UN SDGs

Keywords

Access to Document

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