Abstract
The capsid protein (CP) of Tomato yellow leaf curl virus-Israel (TYLCV-IL), encoded by the v1 gene, is the only known component of the viral capsid. Three point mutations introduced into the conserved NLS region of the CP were investigated. One mutant, in which the Arg at position 19 was converted to Leu, had the most significant effect on the CP-CP homotypic interaction as well as on CP's interaction with its nuclear receptor karyopherin α1 and with the protein GroEL. The latter has been suggested to protect the virions in the insect vector hemolymph. These effects were first observed by yeast two-hybrid assay and then confirmed in tobacco protoplasts by measuring fluorescence resonance energy transfer (FRET) between YFP- and CFP-tagged proteins. Most importantly, when the point mutation converting Arg 19 to Leu was introduced into the full-length TYLCV genome, it disrupted its ability to cause symptoms.
Original language | English |
---|---|
Pages (from-to) | 8-11 |
Number of pages | 4 |
Journal | Virus Research |
Volume | 158 |
Issue number | 1-2 |
DOIs | |
State | Published - Jun 2011 |
Bibliographical note
Funding Information:This work is supported by a grant from the U.S.–Israel Binational Research and Development Fund (BARD) , the U.S.–Israel Binational Science Foundation (BSF) and the Deutsche Forschungsgemeinschaft (DFG) . This paper is a contribution from the Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel, No. 213/10.
Keywords
- Capsid protein
- Geminivirus
- TYLCV