Editing Anti-DNA B Cells by Vλx

Yijin Li, Yoram Louzoun, Martin Weigert

Research output: Contribution to journalArticlepeer-review

39 Scopus citations


Receptor editing is performed by replacement of Vκ genes that contribute to autoreactivity. In addition, the Cκ locus can be deleted by Vκ rearrangement to intronic or 3′ of Cκ RS sequences (also referred to as κ deletion elements). B cells that delete the Cκ can then express λ light chains. However, the λ locus, either of man or mouse, does not allow V gene replacement. Nor does it appear to be deleted. Therefore, editing of autoreactive λ B cells may require alternative pathways. We have found that in anti-DNA heavy chain transgenic mice (tgs) VH3H9/56R, B cells that express anti-DNA receptors comprised of λ1 in association with an anti-DNA heavy chain often coexpress a κ chain that prevents DNA binding. We speculate that such isotypically included cells may have low anti-DNA receptor densities, a feature that may lead to self-tolerance. Here we describe a mechanism of preventing DNA binding by expression of a rarely used member of the Vλ family, Vλx. The λx B cells of the tgs also express CD25 and may represent B cells that have exhausted light chain editing possibilities.

Original languageEnglish
Pages (from-to)337-346
Number of pages10
JournalJournal of Experimental Medicine
Issue number3
StatePublished - 2 Feb 2004


FundersFunder number
National Institute of General Medical SciencesR37GM020964


    • Autoimmunity
    • CD25
    • Light chain
    • Lupus
    • Myelin basic protein


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