EBNA1 regulates cellular gene expression by binding cellular promoters

Allon Canaan; Izhak Haviv; Alexander E. Urban; Vincent P. Schulz; Steve Hartman; Zhengdong Zhang; Dean Palejev; Albert B. Deisseroth; Jill Lacy; Michael Snyder; Mark Gerstein; Sherman M. Weissman

doi:10.1073/pnas.0911676106

EBNA1 regulates cellular gene expression by binding cellular promoters

Allon Canaan
, Izhak Haviv
, Alexander E. Urban
, Vincent P. Schulz
, Steve Hartman
, Zhengdong Zhang
, Dean Palejev
, Albert B. Deisseroth
, Jill Lacy
, Michael Snyder
, Mark Gerstein
, Sherman M. Weissman

Research output: Contribution to journal › Article › peer-review

87 Scopus citations

Abstract

Epstein - Barr virus (EBV) is associated with several types of lymphomas and epithelial tumors including Burkitt's lymphoma (BL), HIV-associated lymphoma, posttransplant lymphoproliferative disorder, and nasopharyngeal carcinoma. EBV nuclear antigen 1 (EBNA1) is expressed in all EBV associated tumors and is required for latency and transformation. EBNA1 initiates latent viral replication in B cells, maintains the viral genome copy number, and regulates transcription of other EBV-encoded latent genes. These activities are mediated through the ability of EBNA1 to bind viral-DNA. To further elucidate the role of EBNA1 in the host cell, we have examined the effect of EBNA1 on cellular gene expression by microarray analysis using the B cell BJAB and the epithelial 293 cell lines transfected with EBNA1. Analysis of the data revealed distinct profiles of cellular gene changes in BJAB and 293 cell lines. Subsequently, chromatin immuneprecipitation revealed a direct binding ofEBNA1to cellular promoters. We have correlated EBNA1 bound promoters with changes in gene expression. Sequence analysis of the 100 promoters most enriched revealed a DNA motif that differs from the EBNA1 binding site in the EBV genome.

Original language	English
Pages (from-to)	22421-22426
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	106
Issue number	52
DOIs	https://doi.org/10.1073/pnas.0911676106
State	Published - 29 Dec 2009
Externally published	Yes

Funding

Funders	Funder number
National Institute of Diabetes and Digestive and Kidney Diseases	P30DK072442

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1073/pnas.0911676106

Cite this

Canaan, A., Haviv, I., Urban, A. E., Schulz, V. P., Hartman, S., Zhang, Z., Palejev, D., Deisseroth, A. B., Lacy, J., Snyder, M., Gerstein, M., & Weissman, S. M. (2009). EBNA1 regulates cellular gene expression by binding cellular promoters. Proceedings of the National Academy of Sciences of the United States of America, 106(52), 22421-22426. https://doi.org/10.1073/pnas.0911676106

@article{963236816208447682f99cd5812fd347,

title = "EBNA1 regulates cellular gene expression by binding cellular promoters",

abstract = "Epstein - Barr virus (EBV) is associated with several types of lymphomas and epithelial tumors including Burkitt's lymphoma (BL), HIV-associated lymphoma, posttransplant lymphoproliferative disorder, and nasopharyngeal carcinoma. EBV nuclear antigen 1 (EBNA1) is expressed in all EBV associated tumors and is required for latency and transformation. EBNA1 initiates latent viral replication in B cells, maintains the viral genome copy number, and regulates transcription of other EBV-encoded latent genes. These activities are mediated through the ability of EBNA1 to bind viral-DNA. To further elucidate the role of EBNA1 in the host cell, we have examined the effect of EBNA1 on cellular gene expression by microarray analysis using the B cell BJAB and the epithelial 293 cell lines transfected with EBNA1. Analysis of the data revealed distinct profiles of cellular gene changes in BJAB and 293 cell lines. Subsequently, chromatin immuneprecipitation revealed a direct binding ofEBNA1to cellular promoters. We have correlated EBNA1 bound promoters with changes in gene expression. Sequence analysis of the 100 promoters most enriched revealed a DNA motif that differs from the EBNA1 binding site in the EBV genome.",

author = "Allon Canaan and Izhak Haviv and Urban, \{Alexander E.\} and Schulz, \{Vincent P.\} and Steve Hartman and Zhengdong Zhang and Dean Palejev and Deisseroth, \{Albert B.\} and Jill Lacy and Michael Snyder and Mark Gerstein and Weissman, \{Sherman M.\}",

year = "2009",

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doi = "10.1073/pnas.0911676106",

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Canaan, A, Haviv, I, Urban, AE, Schulz, VP, Hartman, S, Zhang, Z, Palejev, D, Deisseroth, AB, Lacy, J, Snyder, M, Gerstein, M & Weissman, SM 2009, 'EBNA1 regulates cellular gene expression by binding cellular promoters', Proceedings of the National Academy of Sciences of the United States of America, vol. 106, no. 52, pp. 22421-22426. https://doi.org/10.1073/pnas.0911676106

TY - JOUR

T1 - EBNA1 regulates cellular gene expression by binding cellular promoters

AU - Canaan, Allon

AU - Haviv, Izhak

AU - Urban, Alexander E.

AU - Schulz, Vincent P.

AU - Hartman, Steve

AU - Zhang, Zhengdong

AU - Palejev, Dean

AU - Deisseroth, Albert B.

AU - Lacy, Jill

AU - Snyder, Michael

AU - Gerstein, Mark

AU - Weissman, Sherman M.

PY - 2009/12/29

Y1 - 2009/12/29

N2 - Epstein - Barr virus (EBV) is associated with several types of lymphomas and epithelial tumors including Burkitt's lymphoma (BL), HIV-associated lymphoma, posttransplant lymphoproliferative disorder, and nasopharyngeal carcinoma. EBV nuclear antigen 1 (EBNA1) is expressed in all EBV associated tumors and is required for latency and transformation. EBNA1 initiates latent viral replication in B cells, maintains the viral genome copy number, and regulates transcription of other EBV-encoded latent genes. These activities are mediated through the ability of EBNA1 to bind viral-DNA. To further elucidate the role of EBNA1 in the host cell, we have examined the effect of EBNA1 on cellular gene expression by microarray analysis using the B cell BJAB and the epithelial 293 cell lines transfected with EBNA1. Analysis of the data revealed distinct profiles of cellular gene changes in BJAB and 293 cell lines. Subsequently, chromatin immuneprecipitation revealed a direct binding ofEBNA1to cellular promoters. We have correlated EBNA1 bound promoters with changes in gene expression. Sequence analysis of the 100 promoters most enriched revealed a DNA motif that differs from the EBNA1 binding site in the EBV genome.

AB - Epstein - Barr virus (EBV) is associated with several types of lymphomas and epithelial tumors including Burkitt's lymphoma (BL), HIV-associated lymphoma, posttransplant lymphoproliferative disorder, and nasopharyngeal carcinoma. EBV nuclear antigen 1 (EBNA1) is expressed in all EBV associated tumors and is required for latency and transformation. EBNA1 initiates latent viral replication in B cells, maintains the viral genome copy number, and regulates transcription of other EBV-encoded latent genes. These activities are mediated through the ability of EBNA1 to bind viral-DNA. To further elucidate the role of EBNA1 in the host cell, we have examined the effect of EBNA1 on cellular gene expression by microarray analysis using the B cell BJAB and the epithelial 293 cell lines transfected with EBNA1. Analysis of the data revealed distinct profiles of cellular gene changes in BJAB and 293 cell lines. Subsequently, chromatin immuneprecipitation revealed a direct binding ofEBNA1to cellular promoters. We have correlated EBNA1 bound promoters with changes in gene expression. Sequence analysis of the 100 promoters most enriched revealed a DNA motif that differs from the EBNA1 binding site in the EBV genome.

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JF - Proceedings of the National Academy of Sciences of the United States of America

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ER -

EBNA1 regulates cellular gene expression by binding cellular promoters

Abstract

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UN SDGs

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