E2fs up-regulate expression of genes involved in DNA replication, DNA repair and mitosis

Shirley Polager, Yael Kalma, Eli Berkovich, Doron Ginsberg

Research output: Contribution to journalArticlepeer-review

230 Scopus citations

Abstract

The E2F family of transcription factors plays a pivotal role in the regulation of cell proliferation in higher eukaryotes. We used DNA microarrays and cell lines containing either inducible E2F-1 or inducible E2F-3 to identify novel E2F target genes. Our data indicate that E2F up-regulates the expression of genes not previously described as E2F target genes. A number of these E2F-regulated genes are involved in DNA replication, DNA repair and mitosis. These results suggest that E2F affects cell cycle progression both at S phase and during mitosis. Furthermore, our findings indicate that E2F-dependent gene activation may contribute to the cellular response to DNA damage.

Original languageEnglish
Pages (from-to)437-446
Number of pages10
JournalOncogene
Volume21
Issue number3
DOIs
StatePublished - 17 Jan 2002
Externally publishedYes

Bibliographical note

Funding Information:
We thank Drs WG Kaelin Jr and PD Adams for Rat-1-MT-E2F-1 cells, Dr Shirley Horn-Saban for excellent technical assistance with DNA microarray analysis, Dr Ron Ophir for assistance with the statistical analysis of the data and Gili Hart for technical assistance. This work was supported in part by grants from the Israel Cancer Research Fund (ICRF), Minerva Foundation (Germany) and Yad Abraham Research Center for Diagnostics and Therapy. D Ginsberg is an incumbent of the Recanati Career Development chair of cancer research.

Keywords

  • Cell cycle
  • DNA microarrays
  • DNA repair
  • DNA replication
  • E2F
  • Mitosis

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