E2fs up-regulate expression of genes involved in DNA replication, DNA repair and mitosis

Shirley Polager; Yael Kalma; Eli Berkovich; Doron Ginsberg

doi:10.1038/sj.onc.1205102

E2fs up-regulate expression of genes involved in DNA replication, DNA repair and mitosis

Shirley Polager, Yael Kalma, Eli Berkovich, Doron Ginsberg

Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

232 Scopus citations

Abstract

The E2F family of transcription factors plays a pivotal role in the regulation of cell proliferation in higher eukaryotes. We used DNA microarrays and cell lines containing either inducible E2F-1 or inducible E2F-3 to identify novel E2F target genes. Our data indicate that E2F up-regulates the expression of genes not previously described as E2F target genes. A number of these E2F-regulated genes are involved in DNA replication, DNA repair and mitosis. These results suggest that E2F affects cell cycle progression both at S phase and during mitosis. Furthermore, our findings indicate that E2F-dependent gene activation may contribute to the cellular response to DNA damage.

Original language	English
Pages (from-to)	437-446
Number of pages	10
Journal	Oncogene
Volume	21
Issue number	3
DOIs	https://doi.org/10.1038/sj.onc.1205102
State	Published - 17 Jan 2002
Externally published	Yes

Bibliographical note

Funding Information:
We thank Drs WG Kaelin Jr and PD Adams for Rat-1-MT-E2F-1 cells, Dr Shirley Horn-Saban for excellent technical assistance with DNA microarray analysis, Dr Ron Ophir for assistance with the statistical analysis of the data and Gili Hart for technical assistance. This work was supported in part by grants from the Israel Cancer Research Fund (ICRF), Minerva Foundation (Germany) and Yad Abraham Research Center for Diagnostics and Therapy. D Ginsberg is an incumbent of the Recanati Career Development chair of cancer research.

Funding

We thank Drs WG Kaelin Jr and PD Adams for Rat-1-MT-E2F-1 cells, Dr Shirley Horn-Saban for excellent technical assistance with DNA microarray analysis, Dr Ron Ophir for assistance with the statistical analysis of the data and Gili Hart for technical assistance. This work was supported in part by grants from the Israel Cancer Research Fund (ICRF), Minerva Foundation (Germany) and Yad Abraham Research Center for Diagnostics and Therapy. D Ginsberg is an incumbent of the Recanati Career Development chair of cancer research.

Funders	Funder number
Yad Abraham Research Center for Diagnostics and Therapy
Israel Cancer Research Fund
Minerva Foundation

Keywords

Cell cycle
DNA microarrays
DNA repair
DNA replication
E2F
Mitosis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/sj.onc.1205102

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abstract = "The E2F family of transcription factors plays a pivotal role in the regulation of cell proliferation in higher eukaryotes. We used DNA microarrays and cell lines containing either inducible E2F-1 or inducible E2F-3 to identify novel E2F target genes. Our data indicate that E2F up-regulates the expression of genes not previously described as E2F target genes. A number of these E2F-regulated genes are involved in DNA replication, DNA repair and mitosis. These results suggest that E2F affects cell cycle progression both at S phase and during mitosis. Furthermore, our findings indicate that E2F-dependent gene activation may contribute to the cellular response to DNA damage.",

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E2fs up-regulate expression of genes involved in DNA replication, DNA repair and mitosis

Abstract

Bibliographical note

Funding

Keywords

UN SDGs

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