Dynamic molecular interactions linking the T cell antigen receptor to the actin cytoskeleton

Mira Barda-Saad; Alex Braiman; Rachel Titerence; Stephen C. Bunnell; Valarie A. Barr; Lawrence E. Samelson

doi:10.1038/ni1143

Dynamic molecular interactions linking the T cell antigen receptor to the actin cytoskeleton

Mira Barda-Saad, Alex Braiman, Rachel Titerence, Stephen C. Bunnell, Valarie A. Barr, Lawrence E. Samelson

Research output: Contribution to journal › Article › peer-review

267 Scopus citations

Abstract

T cell receptor (TCR) engagement leads to actin polymerization at the site of T cell contact with antigen-presenting cells. Here we have studied the dynamic activity of proteins involved in regulating actin polymerization in live T cells after activation. Two such adaptor proteins, Nck and the Wiskott-Aldrich syndrome protein (WASp), were recruited to the TCR during initial T cell activation, where they colocalized with the tyrosine kinase Zap70. The recruitment of Nck and WASp depended on TCR-induced tyrosine phosphorylation and the LAT and SLP-76 adaptors. Nck and WASp migrated peripherally and accumulated at an actin-rich circumferential ring. Thus, actin polymerization regulated by the TCR begins at the TCR. Molecules recruited to the TCR regulate actin polymerization and this process drives plasma membrane movement and cellular spreading.

Original language	English
Pages (from-to)	80-89
Number of pages	10
Journal	Nature Immunology
Volume	6
Issue number	1
DOIs	https://doi.org/10.1038/ni1143
State	Published - Jan 2005
Externally published	Yes

Bibliographical note

Funding Information:
The authors thank S. Saitoh and D. Mahadeo for their help; S. Garfield and S. Wincovitch for their help in the operation of the LSM 510; B. Taylor for cell sorting; and P. Schwartzberg and C. Sommers for reading the manuscript. Supported by the Cancer Research Institute (S.C.B.) and the Office of Research on Women’s Health, Foundation for Advanced Education in the Sciences, National Institutes of Health (R.T.).

Funding

The authors thank S. Saitoh and D. Mahadeo for their help; S. Garfield and S. Wincovitch for their help in the operation of the LSM 510; B. Taylor for cell sorting; and P. Schwartzberg and C. Sommers for reading the manuscript. Supported by the Cancer Research Institute (S.C.B.) and the Office of Research on Women’s Health, Foundation for Advanced Education in the Sciences, National Institutes of Health (R.T.).

Funders	Funder number
Office of Research on Women’s Health, Foundation for Advanced Education in the Sciences
National Institutes of Health
National Cancer Institute	Z01BC010304
Cancer research institute

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title = "Dynamic molecular interactions linking the T cell antigen receptor to the actin cytoskeleton",

abstract = "T cell receptor (TCR) engagement leads to actin polymerization at the site of T cell contact with antigen-presenting cells. Here we have studied the dynamic activity of proteins involved in regulating actin polymerization in live T cells after activation. Two such adaptor proteins, Nck and the Wiskott-Aldrich syndrome protein (WASp), were recruited to the TCR during initial T cell activation, where they colocalized with the tyrosine kinase Zap70. The recruitment of Nck and WASp depended on TCR-induced tyrosine phosphorylation and the LAT and SLP-76 adaptors. Nck and WASp migrated peripherally and accumulated at an actin-rich circumferential ring. Thus, actin polymerization regulated by the TCR begins at the TCR. Molecules recruited to the TCR regulate actin polymerization and this process drives plasma membrane movement and cellular spreading.",

author = "Mira Barda-Saad and Alex Braiman and Rachel Titerence and Bunnell, {Stephen C.} and Barr, {Valarie A.} and Samelson, {Lawrence E.}",

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AU - Titerence, Rachel

AU - Bunnell, Stephen C.

AU - Barr, Valarie A.

AU - Samelson, Lawrence E.

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Dynamic molecular interactions linking the T cell antigen receptor to the actin cytoskeleton

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