Dynamic hyper-editing underlies temperature adaptation in Drosophila

Ilana Buchumenski, Osnat Bartok, Reut Ashwal-Fluss, Varun Pandey, Hagit T. Porath, Erez Y. Levanon, Sebastian Kadener

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

In Drosophila, A-to-I editing is prevalent in the brain, and mutations in the editing enzyme ADAR correlate with specific behavioral defects. Here we demonstrate a role for ADAR in behavioral temperature adaptation in Drosophila. Although there is a higher level of editing at lower temperatures, at 29°C more sites are edited. These sites are less evolutionarily conserved, more disperse, less likely to be involved in secondary structures, and more likely to be located in exons. Interestingly, hypomorph mutants for ADAR display a weaker transcriptional response to temperature changes than wild-type flies and a highly abnormal behavioral response upon temperature increase. In sum, our data shows that ADAR is essential for proper temperature adaptation, a key behavior trait that is essential for survival of flies in the wild. Moreover, our results suggest a more general role of ADAR in regulating RNA secondary structures in vivo.

Original languageEnglish
Article numbere1006931
JournalPLoS Genetics
Volume13
Issue number7
DOIs
StatePublished - Jul 2017

Bibliographical note

Publisher Copyright:
© 2017 Buchumenski et al.

Fingerprint

Dive into the research topics of 'Dynamic hyper-editing underlies temperature adaptation in Drosophila'. Together they form a unique fingerprint.

Cite this