Duality of the carbohydrate-recognition system of Pseudomonas aeruginosa-II lectin (PA-IIL)

Albert M. Wu; Yu Ping Gong; Chia Chen Li; Nechama Gilboa-Garber

doi:10.1016/j.febslet.2010.04.026

Duality of the carbohydrate-recognition system of Pseudomonas aeruginosa-II lectin (PA-IIL)

Albert M. Wu
, Yu Ping Gong
, Chia Chen Li
, Nechama Gilboa-Garber

The Mina and Everard Goodman Faculty of Life Sciences - at Bar-Ilan University

Chang Gung University

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

The study of Pseudomonas aeruginosa-II lectin (PA-IIL) complexes with Man derivatives as a recognition factor has been neglected since its monomer is a very weak ligand. Here, the roles of Man oligomers and complexes in PA-IIL carbohydrate-recognition were studied by both enzyme-linked lectinosorbent and inhibition assays. From the results obtained, it is proposed that high density weak -OH conformation as seen in yeast mannan is also an important PA-IIL recognition factor. This finding provides a peculiar concept of the duality of PA-IIL recognition system for lFucα1→ and related complexes and for high density Manα1→ complexes present in polymannosylated target macromolecules.

Original language	English
Pages (from-to)	2371-2375
Number of pages	5
Journal	FEBS Letters
Volume	584
Issue number	11
DOIs	https://doi.org/10.1016/j.febslet.2010.04.026
State	Published - 3 Jun 2010

Bibliographical note

Funding Information:
This work was supported by Grants from the Chang-Gung Medical Research Project (CMRP Nos. 180481 and 170442 ), Kwei-san, Tao-yuan, Taiwan, the National Science Council (NSC 97-2628-B-182-002-MY3 and 97-2320-B-182-020-MY3 ), Taipei, Taiwan.

Funding

This work was supported by Grants from the Chang-Gung Medical Research Project (CMRP Nos. 180481 and 170442 ), Kwei-san, Tao-yuan, Taiwan, the National Science Council (NSC 97-2628-B-182-002-MY3 and 97-2320-B-182-020-MY3 ), Taipei, Taiwan.

Funders	Funder number
CMRP	170442, 180481
Chang-Gung Medical Research Project
National Science Council	97-2320-B-182-020-MY3, NSC 97-2628-B-182-002-MY3

Keywords

Bacterial lectin
Carbohydrate-recognition
Glycoprotein
N-glycan
Pseudomonas aeruginosa
Yeast mannan

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10.1016/j.febslet.2010.04.026

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title = "Duality of the carbohydrate-recognition system of Pseudomonas aeruginosa-II lectin (PA-IIL)",

abstract = "The study of Pseudomonas aeruginosa-II lectin (PA-IIL) complexes with Man derivatives as a recognition factor has been neglected since its monomer is a very weak ligand. Here, the roles of Man oligomers and complexes in PA-IIL carbohydrate-recognition were studied by both enzyme-linked lectinosorbent and inhibition assays. From the results obtained, it is proposed that high density weak -OH conformation as seen in yeast mannan is also an important PA-IIL recognition factor. This finding provides a peculiar concept of the duality of PA-IIL recognition system for lFucα1→ and related complexes and for high density Manα1→ complexes present in polymannosylated target macromolecules.",

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note = "Funding Information: This work was supported by Grants from the Chang-Gung Medical Research Project (CMRP Nos. 180481 and 170442 ), Kwei-san, Tao-yuan, Taiwan, the National Science Council (NSC 97-2628-B-182-002-MY3 and 97-2320-B-182-020-MY3 ), Taipei, Taiwan.",

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AU - Gong, Yu Ping

AU - Li, Chia Chen

AU - Gilboa-Garber, Nechama

N1 - Funding Information: This work was supported by Grants from the Chang-Gung Medical Research Project (CMRP Nos. 180481 and 170442 ), Kwei-san, Tao-yuan, Taiwan, the National Science Council (NSC 97-2628-B-182-002-MY3 and 97-2320-B-182-020-MY3 ), Taipei, Taiwan.

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N2 - The study of Pseudomonas aeruginosa-II lectin (PA-IIL) complexes with Man derivatives as a recognition factor has been neglected since its monomer is a very weak ligand. Here, the roles of Man oligomers and complexes in PA-IIL carbohydrate-recognition were studied by both enzyme-linked lectinosorbent and inhibition assays. From the results obtained, it is proposed that high density weak -OH conformation as seen in yeast mannan is also an important PA-IIL recognition factor. This finding provides a peculiar concept of the duality of PA-IIL recognition system for lFucα1→ and related complexes and for high density Manα1→ complexes present in polymannosylated target macromolecules.

AB - The study of Pseudomonas aeruginosa-II lectin (PA-IIL) complexes with Man derivatives as a recognition factor has been neglected since its monomer is a very weak ligand. Here, the roles of Man oligomers and complexes in PA-IIL carbohydrate-recognition were studied by both enzyme-linked lectinosorbent and inhibition assays. From the results obtained, it is proposed that high density weak -OH conformation as seen in yeast mannan is also an important PA-IIL recognition factor. This finding provides a peculiar concept of the duality of PA-IIL recognition system for lFucα1→ and related complexes and for high density Manα1→ complexes present in polymannosylated target macromolecules.

KW - Bacterial lectin

KW - Carbohydrate-recognition

KW - Glycoprotein

KW - N-glycan

KW - Pseudomonas aeruginosa

KW - Yeast mannan

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SN - 0014-5793

VL - 584

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EP - 2375

JO - FEBS Letters

JF - FEBS Letters

IS - 11

ER -

Duality of the carbohydrate-recognition system of Pseudomonas aeruginosa-II lectin (PA-IIL)

Abstract

Bibliographical note

Funding

Keywords

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