Duality of the carbohydrate-recognition system of Pseudomonas aeruginosa-II lectin (PA-IIL)

Albert M. Wu, Yu Ping Gong, Chia Chen Li, Nechama Gilboa-Garber

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10 Scopus citations

Abstract

The study of Pseudomonas aeruginosa-II lectin (PA-IIL) complexes with Man derivatives as a recognition factor has been neglected since its monomer is a very weak ligand. Here, the roles of Man oligomers and complexes in PA-IIL carbohydrate-recognition were studied by both enzyme-linked lectinosorbent and inhibition assays. From the results obtained, it is proposed that high density weak -OH conformation as seen in yeast mannan is also an important PA-IIL recognition factor. This finding provides a peculiar concept of the duality of PA-IIL recognition system for lFucα1→ and related complexes and for high density Manα1→ complexes present in polymannosylated target macromolecules.

Original languageEnglish
Pages (from-to)2371-2375
Number of pages5
JournalFEBS Letters
Volume584
Issue number11
DOIs
StatePublished - 3 Jun 2010

Bibliographical note

Funding Information:
This work was supported by Grants from the Chang-Gung Medical Research Project (CMRP Nos. 180481 and 170442 ), Kwei-san, Tao-yuan, Taiwan, the National Science Council (NSC 97-2628-B-182-002-MY3 and 97-2320-B-182-020-MY3 ), Taipei, Taiwan.

Funding

This work was supported by Grants from the Chang-Gung Medical Research Project (CMRP Nos. 180481 and 170442 ), Kwei-san, Tao-yuan, Taiwan, the National Science Council (NSC 97-2628-B-182-002-MY3 and 97-2320-B-182-020-MY3 ), Taipei, Taiwan.

FundersFunder number
CMRP170442, 180481
Chang-Gung Medical Research Project
National Science Council97-2320-B-182-020-MY3, NSC 97-2628-B-182-002-MY3

    Keywords

    • Bacterial lectin
    • Carbohydrate-recognition
    • Glycoprotein
    • N-glycan
    • Pseudomonas aeruginosa
    • Yeast mannan

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