Abstract
The study of Pseudomonas aeruginosa-II lectin (PA-IIL) complexes with Man derivatives as a recognition factor has been neglected since its monomer is a very weak ligand. Here, the roles of Man oligomers and complexes in PA-IIL carbohydrate-recognition were studied by both enzyme-linked lectinosorbent and inhibition assays. From the results obtained, it is proposed that high density weak -OH conformation as seen in yeast mannan is also an important PA-IIL recognition factor. This finding provides a peculiar concept of the duality of PA-IIL recognition system for lFucα1→ and related complexes and for high density Manα1→ complexes present in polymannosylated target macromolecules.
Original language | English |
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Pages (from-to) | 2371-2375 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 584 |
Issue number | 11 |
DOIs | |
State | Published - Jun 2010 |
Bibliographical note
Funding Information:This work was supported by Grants from the Chang-Gung Medical Research Project (CMRP Nos. 180481 and 170442 ), Kwei-san, Tao-yuan, Taiwan, the National Science Council (NSC 97-2628-B-182-002-MY3 and 97-2320-B-182-020-MY3 ), Taipei, Taiwan.
Keywords
- Bacterial lectin
- Carbohydrate-recognition
- Glycoprotein
- N-glycan
- Pseudomonas aeruginosa
- Yeast mannan