The study of Pseudomonas aeruginosa-II lectin (PA-IIL) complexes with Man derivatives as a recognition factor has been neglected since its monomer is a very weak ligand. Here, the roles of Man oligomers and complexes in PA-IIL carbohydrate-recognition were studied by both enzyme-linked lectinosorbent and inhibition assays. From the results obtained, it is proposed that high density weak -OH conformation as seen in yeast mannan is also an important PA-IIL recognition factor. This finding provides a peculiar concept of the duality of PA-IIL recognition system for lFucα1→ and related complexes and for high density Manα1→ complexes present in polymannosylated target macromolecules.
|Number of pages||5|
|State||Published - Jun 2010|
Bibliographical noteFunding Information:
This work was supported by Grants from the Chang-Gung Medical Research Project (CMRP Nos. 180481 and 170442 ), Kwei-san, Tao-yuan, Taiwan, the National Science Council (NSC 97-2628-B-182-002-MY3 and 97-2320-B-182-020-MY3 ), Taipei, Taiwan.
- Bacterial lectin
- Pseudomonas aeruginosa
- Yeast mannan