Dose-Dependent Transcriptional Response to Ionizing Radiation Is Orchestrated with DNA Repair within the Nuclear Space

Garima Chaturvedi, Avital Sarusi-Portuguez, Olga Loza, Ariel Shimoni-Sebag, Orly Yoron, Yaacov Richard Lawrence, Leor Zach, Ofir Hakim

Research output: Contribution to journalArticlepeer-review


Radiation therapy is commonly used to treat glioblastoma multiforme (GBM) brain tumors. Ionizing radiation (IR) induces dose-specific variations in transcriptional programs, implicating that they are tightly regulated and critical components in the tumor response and survival. Yet, our understanding of the downstream molecular events triggered by effective vs. non-effective IR doses is limited. Herein, we report that variations in the genetic programs are positively and functionally correlated with the exposure to effective or non-effective IR doses. Genome architecture analysis revealed that gene regulation is spatially and temporally coordinated with DNA repair kinetics. The radiation-activated genes were pre-positioned in active sub-nuclear compartments and were upregulated following the DNA damage response, while the DNA repair activity shifted to the inactive heterochromatic spatial compartments. The IR dose affected the levels of DNA damage repair and transcription modulation, but not the order of the events, which was linked to their spatial nuclear positioning. Thus, the distinct coordinated temporal dynamics of DNA damage repair and transcription reprogramming in the active and inactive sub-nuclear compartments highlight the importance of high-order genome organization in synchronizing the molecular events following IR.

Original languageEnglish
Article number970
JournalInternational Journal of Molecular Sciences
Issue number2
StatePublished - 12 Jan 2024

Bibliographical note

Publisher Copyright:
© 2024 by the authors.


  • DNA damage response
  • GBM
  • TADs
  • genome architecture
  • ionizing radiation
  • transcription


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