Dopamine receptors in the rat entopeduncular nucleus

Hagar Lavian, Yocheved Loewenstern, Ravit Madar, Mara Almog, Izhar Bar-Gad, Eitan Okun, Alon Korngreen

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Dopamine is critical for the normal functioning of the basal ganglia, modulating both input and output nuclei of this system. The distribution and function of each of the five dopamine receptor subtypes have been studied extensively in the striatum. However, the role of extrastriatal dopamine receptors in basal ganglia information processing is less clear. Here, we studied the anatomical distribution of dopamine receptors in one of the output nuclei of the rodent basal ganglia, the entopeduncular nucleus (EP). The presence of all dopamine receptor subtypes was verified in the EP using immunostaining. We detected co-localization of dopamine receptors with VGAT, which suggests presynaptic expression on GABAergic terminals. D1R and D2R were strongly colocalized with VGAT, whereas DR3-5 showed only sparse co-localization. We further labeled striatal or pallidal neurons with GFP and showed that only D1 receptors were co-localized with striatal terminals, while only D2R and D3R were co-localized with pallidal terminals. Dopamine receptors were also strongly co-localized with MAP2, indicating postsynaptic expression. Overall, these findings suggest that the dopaminergic system modulates activity in the EP both directly via postsynaptic receptors, and indirectly via GABAergic synapses stemming from the direct and indirect pathways.

Original languageEnglish
Pages (from-to)2673-2684
Number of pages12
JournalBrain Structure and Function
Volume223
Issue number6
Early online date22 Mar 2018
DOIs
StatePublished - 1 Jul 2018

Bibliographical note

Publisher Copyright:
© 2018, Springer-Verlag GmbH Germany, part of Springer Nature.

Keywords

  • Basal ganglia
  • Dopamine receptors
  • Entopeduncular nucleus
  • Globus pallidus
  • Striatum

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