TY - JOUR
T1 - Dopamine increases glial cell line-derived neurotrophic factor in human fetal astrocytes
AU - Kinor, Noa
AU - Geffen, Revital
AU - Golomb, Eliahu
AU - Zinman, Tova
AU - Yadid, Gal
PY - 2001/2
Y1 - 2001/2
N2 - The use of fetal astrocytes for gene delivery into brains with neurodegenerative diseases has been suggested. Therefore, the effects of neurotransmitters in the brain on such cells are of interest. The presence of D1 (D1A) receptors and the effect of dopamine on a fetal human astrocyte cell line (SVG cells) in vitro were examined. SVG cells expressed D1 (D1A), but not D5 (D1B) receptors, as shown by RT-PCR. Exposure to dopamine, apomorphine, and the specific D1 agonist, SKF-38393, increased glial-derived neurotrophic factor production of SVG cells, as well as intracellular free calcium. Exposure to the specific D1 antagonist, SCH 23390, blocked these effects. Thus, if implanted into a brain region rich in dopamine, or if transfected with the tyrosine hydroxylase gene, fetal astrocytes may serve as paracrine/autocrine cells capable of supplying critical growth factors to diseased brain tissue.
AB - The use of fetal astrocytes for gene delivery into brains with neurodegenerative diseases has been suggested. Therefore, the effects of neurotransmitters in the brain on such cells are of interest. The presence of D1 (D1A) receptors and the effect of dopamine on a fetal human astrocyte cell line (SVG cells) in vitro were examined. SVG cells expressed D1 (D1A), but not D5 (D1B) receptors, as shown by RT-PCR. Exposure to dopamine, apomorphine, and the specific D1 agonist, SKF-38393, increased glial-derived neurotrophic factor production of SVG cells, as well as intracellular free calcium. Exposure to the specific D1 antagonist, SCH 23390, blocked these effects. Thus, if implanted into a brain region rich in dopamine, or if transfected with the tyrosine hydroxylase gene, fetal astrocytes may serve as paracrine/autocrine cells capable of supplying critical growth factors to diseased brain tissue.
KW - Cell transplantation
KW - D receptors
KW - D receptors
KW - Glial cell line-derived neurotrophic factor
KW - Human astrocytes
KW - Intracellular calcium
UR - http://www.scopus.com/inward/record.url?scp=0035131340&partnerID=8YFLogxK
U2 - 10.1002/1098-1136(200102)33:2<143::AID-GLIA1013>3.0.CO;2-3
DO - 10.1002/1098-1136(200102)33:2<143::AID-GLIA1013>3.0.CO;2-3
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C2 - 11180511
AN - SCOPUS:0035131340
SN - 0894-1491
VL - 33
SP - 143
EP - 150
JO - GLIA
JF - GLIA
IS - 2
ER -
