TY - JOUR
T1 - DNA Microspheres Coated with Bioavailable Polymer as an Efficient Gene Expression Agent in Yeasts
AU - Reytblat, Irena
AU - Lipovsky, Anat
AU - Gedanken, Aharon
N1 - Publisher Copyright:
Copyright © 2016 Irena Reytblat et al.
PY - 2016
Y1 - 2016
N2 - Gene delivery is one of the steps necessary for gene therapy and for genetic modification. However, delivering DNA into cells is challenging due to its negative charge that leads to repulsion by the negative cell membrane. In the current research, DNA spheres with a DNA encoding to a certain gene were coated with bioavailable polymers, polyethylene imine (PEI) and polycaprolactone (PCL), in a short, one-step sonochemical reaction. The polymers were used in order to neutralize the negative charge of the DNA. Our study shows that the DNA nanospheres not only managed to penetrate the cell without causing it any damage, but also expressed the desired gene inside it.
AB - Gene delivery is one of the steps necessary for gene therapy and for genetic modification. However, delivering DNA into cells is challenging due to its negative charge that leads to repulsion by the negative cell membrane. In the current research, DNA spheres with a DNA encoding to a certain gene were coated with bioavailable polymers, polyethylene imine (PEI) and polycaprolactone (PCL), in a short, one-step sonochemical reaction. The polymers were used in order to neutralize the negative charge of the DNA. Our study shows that the DNA nanospheres not only managed to penetrate the cell without causing it any damage, but also expressed the desired gene inside it.
UR - http://www.scopus.com/inward/record.url?scp=84958068948&partnerID=8YFLogxK
U2 - 10.1155/2016/5178029
DO - 10.1155/2016/5178029
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AN - SCOPUS:84958068948
SN - 1687-4110
VL - 2016
JO - Journal of Nanomaterials
JF - Journal of Nanomaterials
M1 - 5178029
ER -