DNA Microspheres Coated with Bioavailable Polymer as an Efficient Gene Expression Agent in Yeasts

Irena Reytblat, Anat Lipovsky, Aharon Gedanken

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Gene delivery is one of the steps necessary for gene therapy and for genetic modification. However, delivering DNA into cells is challenging due to its negative charge that leads to repulsion by the negative cell membrane. In the current research, DNA spheres with a DNA encoding to a certain gene were coated with bioavailable polymers, polyethylene imine (PEI) and polycaprolactone (PCL), in a short, one-step sonochemical reaction. The polymers were used in order to neutralize the negative charge of the DNA. Our study shows that the DNA nanospheres not only managed to penetrate the cell without causing it any damage, but also expressed the desired gene inside it.

Original languageEnglish
Article number5178029
JournalJournal of Nanomaterials
Volume2016
DOIs
StatePublished - 2016

Bibliographical note

Publisher Copyright:
Copyright © 2016 Irena Reytblat et al.

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