DNA chip-based diagnosis of onychomycosis and tinea pedis

Katja Bieber, Melanie Harder, Sascha Ständer, Katharina Boch, Khalaf Kridin, Birgit Köhler, Waltraud Anemüller, Anna Lara Ernst, Detlef Zillikens, Markus Cavalar, Ralf J. Ludwig

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Background and objectives: Onychomycosis (OM) and tinea pedis (TP) are common fungal infections. Currently, diagnosis is based on direct microscopy and culture that have a low to moderate sensitivity and/or require up to 3–4 weeks until results are obtained. PCR techniques have emerged for the diagnosis of fungal infections, but little is known about their sensitivity and specificity in diagnosing. Here, we compared the diagnostic value of a DNA-chip technology, that detects 56 fungal pathogens, in a single-center prospective diagnostic study with microscopy and culture in suspected OM/TP. Patients and methods: Microscopy, culture and DNA microarray assays were performed on scraping material from patients with suspected OM (n = 67) or TP (n = 73). To test whether swabs can be used as an alternative for scraping, PCR yields were compared in a further 13 patients with OM and 11 patients with TP. Results: DNA microarrays had the highest sensitivity. Combination of DNA-chip technology with microscopy further increased the sensitivity, and results from this combined laboratory diagnosis can be obtained within 24 hours. Comparison of sampling techniques (scraping, dry or wet swab) for DNA-chip assays showed similar results in suspected OM or TP. Conclusions: DNA-chip technology shows high sensitivity for OM and TP diagnosis, especially when combined with microscopy.

Original languageEnglish
Pages (from-to)1112-1121
Number of pages10
JournalJDDG - Journal of the German Society of Dermatology
Volume20
Issue number8
DOIs
StatePublished - Aug 2022

Bibliographical note

Publisher Copyright:
© 2022 The Authors. Journal der Deutschen Dermatologischen Gesellschaft published by John Wiley & Sons Ltd on behalf of Deutsche Dermatologische Gesellschaft.

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