Direct spectrophotometric determination of inorganic sulfide in biological materials and in other complex mixtures

Nechama Gilboa-Garber

Research output: Contribution to journalArticlepeer-review

141 Scopus citations

Abstract

An improved direct spectrophotometric method is described that is suited to determinations of sulfide in enzyme mixtures and in biological materials. The assay is based on the specific incorporation of sulfide into methylene blue in the presence of acidic, N,N-dimethyl-p-phenylenedi-amine and ferric chloride. The major improvement in the method is achieved by quantitative precipitation of zinc sulfide and removal of the soluble components of the sample. This procedure replaces the cumbersome entrainment of hydrogen sulfide with nitrogen gas prior to addition of the color-forming reagents. This very simple in situ manipulation is efficient in preventing the interfering effects of foreign constituents of the sample medium and contributes to a further increase in the sensitivity of the method.

Original languageEnglish
Pages (from-to)129-133
Number of pages5
JournalAnalytical Biochemistry
Volume43
Issue number1
DOIs
StatePublished - Sep 1971

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