TY - JOUR
T1 - Direct spectrophotometric determination of inorganic sulfide in biological materials and in other complex mixtures
AU - Gilboa-Garber, Nechama
PY - 1971/9
Y1 - 1971/9
N2 - An improved direct spectrophotometric method is described that is suited to determinations of sulfide in enzyme mixtures and in biological materials. The assay is based on the specific incorporation of sulfide into methylene blue in the presence of acidic, N,N-dimethyl-p-phenylenedi-amine and ferric chloride. The major improvement in the method is achieved by quantitative precipitation of zinc sulfide and removal of the soluble components of the sample. This procedure replaces the cumbersome entrainment of hydrogen sulfide with nitrogen gas prior to addition of the color-forming reagents. This very simple in situ manipulation is efficient in preventing the interfering effects of foreign constituents of the sample medium and contributes to a further increase in the sensitivity of the method.
AB - An improved direct spectrophotometric method is described that is suited to determinations of sulfide in enzyme mixtures and in biological materials. The assay is based on the specific incorporation of sulfide into methylene blue in the presence of acidic, N,N-dimethyl-p-phenylenedi-amine and ferric chloride. The major improvement in the method is achieved by quantitative precipitation of zinc sulfide and removal of the soluble components of the sample. This procedure replaces the cumbersome entrainment of hydrogen sulfide with nitrogen gas prior to addition of the color-forming reagents. This very simple in situ manipulation is efficient in preventing the interfering effects of foreign constituents of the sample medium and contributes to a further increase in the sensitivity of the method.
UR - http://www.scopus.com/inward/record.url?scp=0015115637&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(71)90116-3
DO - 10.1016/0003-2697(71)90116-3
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C2 - 4331757
AN - SCOPUS:0015115637
SN - 0003-2697
VL - 43
SP - 129
EP - 133
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -