Direct production of glucose from glycogen under microwave irradiation

Miri Klein, Indra Neel Pulidindi, Nina Perkas, Ella Meltzer-Mats, Arie Gruzman, Aharon Gedanken

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


The production of fermentable sugars from renewable sources is a challenge. An attempt was made to exploit glycogen as a potential feedstock for the production of glucose. The microwave-assisted acidic hydrolysis was applied for glycogen decomposition for the first time. The optimal conditions for the hydrolysis reaction (yield of glucose - 62 wt.%) were identified: microwave irradiation time - 10 min and concentration of acid - 1 M HCl. Microwave irradiation has dramatically reduced the reaction time from more than 6 h (at 80°C under an oil bath) to 10 min. 13C NMR spectroscopy was employed to monitor the progress of the hydrolysis reaction. HPLC analysis was employed to evaluate the yield of glucose. Thus, the viability of the use of glycogen as an economically and environmentally benign precursor to the production of glucose has been demonstrated.

Original languageEnglish
Pages (from-to)7262-7267
Number of pages6
JournalRSC Advances
Issue number18
StatePublished - 28 Aug 2012


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