Direct observation of mammalian cell growth and size regulation

Sungmin Son, Amit Tzur, Yaochung Weng, Paul Jorgensen, Jisoo Kim, Marc W. Kirschner, Scott R. Manalis

Research output: Contribution to journalArticlepeer-review

170 Scopus citations

Abstract

We introduce a microfluidic system for simultaneously measuring single-cell mass and cell cycle progression over multiple generations. We use this system to obtain over 1,000 h of growth data from mouse lymphoblast and pro-B-cell lymphoid cell lines. Cell lineage analysis revealed a decrease in the growth rate variability at the G1-S phase transition, which suggests the presence of a growth rate threshold for maintaining size homeostasis.

Original languageEnglish
Pages (from-to)910-912
Number of pages3
JournalNature Methods
Volume9
Issue number9
DOIs
StatePublished - Sep 2012

Bibliographical note

Funding Information:
We thank A. Miyawaki (RIKEN) for the mKO2-hCdt1 and mAG-hGem constructs, M. Stevens (MIT), M. Ginzberg and R. Kafri (Harvard Medical School) for valuable discussions and M. Chung (Harvard Medical School) for assistance with generating the FUCCI-labeled L1210 cells. S.R.M. acknowledges support from US National Cancer Institute contracts Physical Sciences Oncology Center U54CA143874 and R21 CA137695 as well as from the National Institutes of General Medical Sciences (NIGMS) EUREKA R01GM085457. M.W.K. acknowledges support from the NIGMS. S.S. acknowledges support from the Kwanjeong Educational Foundation, South Korea, through a graduate fellowship. Y.W. acknowledges support from a National Science Foundation Graduate Research Fellowship, and A.T. acknowledges support from The Marie Curie International Reintegration Grant PIRG-GA-2010-277062 and the Israeli Centers of Research Excellence (I-CORE) program (Center no. 41/11).

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