TY - JOUR
T1 - Direct measurement of the isomerization barrier of the isolated retinal chromophore
AU - Dilger, Jonathan
AU - Musbat, Lihi
AU - Sheves, Mordechai
AU - Bochenkova, Anastasia V.
AU - Clemmer, David E.
AU - Toker, Yoni
N1 - Publisher Copyright:
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
PY - 2015/4/13
Y1 - 2015/4/13
N2 - Isomerizations of the retinal chromophore were investigated using the IMS-IMS technique. Four different structural features of the chromophore were observed, isolated, excited collisionally, and the resulting isomer and fragment distributions were measured. By establishing the threshold activation voltages for isomerization for each of the reaction pathways, and by measuring the threshold activation voltage for fragmentation, the relative energies of the isomers as well as the energy barriers for isomerization were determined. The energy barrier for a single cis-trans isomerization is (0.64±0.05) eV, which is significantly lower than that observed for the reaction within opsin proteins.
AB - Isomerizations of the retinal chromophore were investigated using the IMS-IMS technique. Four different structural features of the chromophore were observed, isolated, excited collisionally, and the resulting isomer and fragment distributions were measured. By establishing the threshold activation voltages for isomerization for each of the reaction pathways, and by measuring the threshold activation voltage for fragmentation, the relative energies of the isomers as well as the energy barriers for isomerization were determined. The energy barrier for a single cis-trans isomerization is (0.64±0.05) eV, which is significantly lower than that observed for the reaction within opsin proteins.
KW - chromophores
KW - gas-phase reactions
KW - ion mobility spectroscopy
KW - isomerization
KW - proteins
UR - http://www.scopus.com/inward/record.url?scp=84926463195&partnerID=8YFLogxK
U2 - 10.1002/anie.201411894
DO - 10.1002/anie.201411894
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C2 - 25756226
AN - SCOPUS:84926463195
SN - 1433-7851
VL - 54
SP - 4748
EP - 4752
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
IS - 16
ER -