Direct cloning and extended culture of antigen-specific MHC-restricted, proliferating T lymphocytes [17]

Benjamin Sredni, Harley Y. Tse, Ronald H. Schwartz

Research output: Contribution to journalLetterpeer-review

86 Scopus citations

Abstract

The development of antigen-specific, T-cell lines has been a subject of intensive investigation by many laboratories interested in the problems of the nature of the T-cell receptor and the mechanism of major histocompatibility complex (MHC) restriction. We describe here a modification of the soft agar cloning technique which has allowed us to rapidly generate and expand proliferating T-lymphocyte clones directly from murine lymph nodes. Using this technique, we demonstrate that such T-cell clones are antigen specific and require I-A compatible antigen-presenting cells for stimulation.

Original languageEnglish
Pages (from-to)581-583
Number of pages3
JournalNature
Volume283
Issue number5747
DOIs
StatePublished - 7 Feb 1980
Externally publishedYes

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