Diffusion reflection measurements of antibodies conjugated to gold nanoparticles as a method to identify cutaneous squamous cell carcinoma borders

Asaf Olshinka, Dean Ad-El, Elena Didkovski, Shirel Weiss, Rinat Ankri, Nitza Goldenberg-Cohen, Dror Fixler

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Diffusion reflectance spectroscopy measurements targeted with gold nanoparticles (GNPs) can identify residual cutaneous squamous cell carcinoma (SCC) in excision borders. Human SCC specimens were stained with hematoxylin and eosin to identify tumor borders, and reflected onto an unstained deparaffinized section. Diffusion reflection of three sites (normal and SCC) were measured before and after GNPs targeting. Hyperspectral imaging showed a mean of 2.5 sites with tumor per specimen and 1.2 tumor-free (p < 0.05, t-test). GNPs were detected in 25/30 tumor sites (sensitivity 83.3%, false-negative rate 16.6%) and 12/30 non-tumor sites (specificity 60%, false-positive rate 40%). This study verifies the use of nanotechnology in identifying SCC tumor margins. Diffusion reflection scanning has high sensitivity for detecting the residual tumor.

Original languageEnglish
Article number447
JournalMaterials
Volume13
Issue number2
DOIs
StatePublished - 17 Jan 2020

Bibliographical note

Publisher Copyright:
© 2020 by the authors.

Keywords

  • Diffusion reflection
  • Epidermal growth factor receptor
  • Gold nanoparticles
  • Hyperspectral imaging
  • Squamous cell carcinoma

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