Diffusion Reflection and Fluorescence Lifetime Imaging Microscopy Study of Fluorophore-Conjugated Gold Nanoparticles or Nanorods in Solid Phantoms

Dror Fixler, Tsviya Nayhoz, Krishanu Ray

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

In this paper we report the optical properties of fluorescein-conjugated gold nanoparticles (GNPs) in solid phantoms using diffusion reflection (DR) and fluorescence lifetime imaging microscopy (FLIM). The GNPs attached with fluorescein in solution were studied by fluorescence correlation spectroscopy. The intensity decays were recorded to reveal the fluorescence lifetime of fluorescein while in the near-field vicinity of the GNPs. The DR method was used to explore the solid phantoms containing GNPs, indicating the light propagation from the surface of solid phantoms. The resulting DR slopes of the reflected intensity showed the higher the GNP concentration, the bigger the slope. Fluorescence intensity, lifetime, and anisotropy images of solid phantoms were investigated by FLIM. The exploration of optical properties and molecular imaging combined with DR and FLIM methods is a new approach that has not been established until now. The combined DR-FLIM technique is expected to provide discrimination based on unique spectroscopic fingerprints of GNPs that could be utilized for cell imaging. This paper includes a combined study with a variety of methods, which may lead to multimodal imaging for surfaces (by FLIM) and deep penetration (up to cm by the DR) together.

Original languageEnglish
Pages (from-to)900-905
Number of pages6
JournalACS Photonics
Volume1
Issue number9
DOIs
StatePublished - 17 Sep 2014

Bibliographical note

Publisher Copyright:
© 2014 American Chemical Society.

Keywords

  • biomolecular imaging
  • diffusion reflection
  • fluorescence anisotropy
  • fluorescence lifetime imaging
  • gold nanoparticles
  • noninvasive detection

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