Differential inhibition of LINE1 and LINE2 retrotransposition by vertebrate AID/APOBEC proteins

Nataša Lindič, Maruška Budič, Toni Petan, Binyamin A. Knisbacher, Erez Y. Levanon, Nika Lovšin

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Background: The role of AID/APOBEC proteins in the mammalian immune response against retroviruses and retrotransposons is well established. G to A hypermutations, the hallmark of their cytidine deaminase activity, are present in several mammalian retrotransposons. However, the role of AID/APOBEC proteins in non-mammalian retroelement restriction is not completely understood.Results: Here we provide the first evidence of anti-retroelement activity of a reptilian APOBEC protein. The green anole lizard A1 protein displayed potent DNA mutator activity and inhibited ex vivo retrotransposition of LINE1 and LINE2 ORF1 protein encoding elements, displaying a mechanism of action similar to that of the human A1 protein. In contrast, the human A3 proteins did not require ORF1 protein to inhibit LINE retrotransposition, suggesting a differential mechanism of anti-LINE action of A1 proteins, which emerged in amniotes, and A3 proteins, exclusive to placental mammals. In accordance, genomic analyses demonstrate differential G to A DNA editing of LINE retrotransposons in the lizard genome, which is also the first evidence for G to A DNA editing in non-mammalian genomes.Conclusion: Our data suggest that vertebrate APOBEC proteins differentially inhibit the retrotransposition of LINE elements and that the anti-retroelement activity of APOBEC proteins predates mammals.

Original languageEnglish
Article number156
Pages (from-to)156
JournalRetrovirology
Volume10
Issue number1
DOIs
StatePublished - 17 Dec 2013

Bibliographical note

Funding Information:
We thank Shai Carmi for his help at the early stage of the project, and Norihiro Okada, John Moran, Bryan Cullen, Boris Matija Peterlin, Rai Kunal, Silvestro Conticello and Yong-Hui Zheng for reagents. We thank Alenka Čopič for critical reading of the manuscript. This work was supported by the Slovenian Research Agency [31952 to NLi, J7-2230, J1-2141 to NLo and MB, J3-4168 to MB and P1-0207 to NLo and TP]. BAK and EYL were supported by the Israel Science foundation [728/10] and by the European Research Council [311257]. Funding for open access charge: 31952 to NLi.

Keywords

  • AID
  • APOBEC3
  • Cytidine deaminase
  • G to A hypermutation
  • LINE1
  • Lizard APOBEC1
  • ORF1p
  • Retrotransposon
  • Zebrafish APOBEC2
  • Zebrafish LINE2

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