The Islet-1 antigen is an early marker of differentiation of neural tube cells, and is expressed in many other embryonic cells as well. It had been reported that Islet-1 is expressed only in post-mitotic sympathetic neuroblasts in vitro, unlike other differentiation markers. We have double- labeled St. 23 chick embryos for bromodeoxyuridine (BrDU) and Islet-1 and found that neural tube and dorsal root ganglion (DRG) cells express Islet-1 after leaving the cell cycle, while sympathetic ganglion (SG) cells express Islet-1 while still dividing.
Bibliographical noteFunding Information:
Thanks are due to Dr. Benny Motro for his critical reading of the manuscript and Dr. Leonid Roitman for help with confocal microscopy. This work was supported by grants from the Dysautonomia Foundation, the Israel Institute for Psychobiology, Charles Smith Foundation and the Health Science Center at Bar-Ilan University, The Aviv Fund for Neuroscience Research. Monoclonal antibodies 40.2D6 (Islet-1) and 4F2 (Lim 2) were developed by T. Jessell and obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained by the University of Iowa, Department of Biological Sciences, Iowa City, IA, 52242.
- Differentiation marker
- Dorsal root ganglia
- Neural crest
- Sympathetic ganglion