Differential effects of interleukin-4 in peptide induced autoimmunity

Bisram Deocharan; Paola Marambio; Morris Edelman; Chaim Putterman

doi:10.1016/s1521-6616(03)00096-2

Differential effects of interleukin-4 in peptide induced autoimmunity

Bisram Deocharan
, Paola Marambio
, Morris Edelman
, Chaim Putterman

Albert Einstein College of Medicine
Winthrop-University Hospital

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

BALB/c mice immunized with multimeric DWEYSVWLSN develop IgG1 anti-DNA antibodies and glomerular immunoglobulin deposits, leading us to investigate the role of IL-4 in this model of antigen induced lupus. Splenocytes from DWEYSVWLSN immunized mice secreted IL-4 but not γ-interferon. Following peptide immunization, IgG1 anti-peptide and anti-DNA antibodies were significantly higher in IL-4 wild type mice, while IgM and IgG3 anti-DNA levels were significantly higher in IL-4 knockout mice. Titers of IgG anti-laminin and anti-histone, but not anti-Sm/RNP and anti-cardiolipin antibodies, were significantly higher in the IL-4 wild type group. Glomerular immunoglobulin deposition was substantially decreased in IL-4 knockout mice. We conclude that while IL-4 does not materially affect the generation of some autoantibody responses associated with peptide induced autoimmunity, IL-4 deficiency inhibits kidney immunoglobulin deposition. The effect of IL-4 on humoral autoimmunity in lupus is complex, and is dependent on genetic background, the antigenic trigger and stage of disease.

Original language	English
Pages (from-to)	80-88
Number of pages	9
Journal	Clinical Immunology
Volume	108
Issue number	2
DOIs	https://doi.org/10.1016/s1521-6616(03)00096-2
State	Published - 1 Aug 2003
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported in part by a National Institute of Health grant RO1 AR48692-01, and an Arthritis Investigator grant from the Arthritis Foundation (to C.P.). We wish to thank Dr. Betty Diamond for many helpful discussions and suggestions.

Funding

This work was supported in part by a National Institute of Health grant RO1 AR48692-01, and an Arthritis Investigator grant from the Arthritis Foundation (to C.P.). We wish to thank Dr. Betty Diamond for many helpful discussions and suggestions.

Funders	Funder number
National Institutes of Health	RO1 AR48692-01
National Institute of Arthritis and Musculoskeletal and Skin Diseases	R01AR048692
Arthritis Foundation

Keywords

Anti-DNA antibodies
Autoantibodies
Cytokines
Glomerulonephritis
Interleukin-4
Peptide mimetope
SLE

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10.1016/s1521-6616(03)00096-2

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title = "Differential effects of interleukin-4 in peptide induced autoimmunity",

abstract = "BALB/c mice immunized with multimeric DWEYSVWLSN develop IgG1 anti-DNA antibodies and glomerular immunoglobulin deposits, leading us to investigate the role of IL-4 in this model of antigen induced lupus. Splenocytes from DWEYSVWLSN immunized mice secreted IL-4 but not γ-interferon. Following peptide immunization, IgG1 anti-peptide and anti-DNA antibodies were significantly higher in IL-4 wild type mice, while IgM and IgG3 anti-DNA levels were significantly higher in IL-4 knockout mice. Titers of IgG anti-laminin and anti-histone, but not anti-Sm/RNP and anti-cardiolipin antibodies, were significantly higher in the IL-4 wild type group. Glomerular immunoglobulin deposition was substantially decreased in IL-4 knockout mice. We conclude that while IL-4 does not materially affect the generation of some autoantibody responses associated with peptide induced autoimmunity, IL-4 deficiency inhibits kidney immunoglobulin deposition. The effect of IL-4 on humoral autoimmunity in lupus is complex, and is dependent on genetic background, the antigenic trigger and stage of disease.",

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note = "Funding Information: This work was supported in part by a National Institute of Health grant RO1 AR48692-01, and an Arthritis Investigator grant from the Arthritis Foundation (to C.P.). We wish to thank Dr. Betty Diamond for many helpful discussions and suggestions.",

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AU - Edelman, Morris

AU - Putterman, Chaim

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AB - BALB/c mice immunized with multimeric DWEYSVWLSN develop IgG1 anti-DNA antibodies and glomerular immunoglobulin deposits, leading us to investigate the role of IL-4 in this model of antigen induced lupus. Splenocytes from DWEYSVWLSN immunized mice secreted IL-4 but not γ-interferon. Following peptide immunization, IgG1 anti-peptide and anti-DNA antibodies were significantly higher in IL-4 wild type mice, while IgM and IgG3 anti-DNA levels were significantly higher in IL-4 knockout mice. Titers of IgG anti-laminin and anti-histone, but not anti-Sm/RNP and anti-cardiolipin antibodies, were significantly higher in the IL-4 wild type group. Glomerular immunoglobulin deposition was substantially decreased in IL-4 knockout mice. We conclude that while IL-4 does not materially affect the generation of some autoantibody responses associated with peptide induced autoimmunity, IL-4 deficiency inhibits kidney immunoglobulin deposition. The effect of IL-4 on humoral autoimmunity in lupus is complex, and is dependent on genetic background, the antigenic trigger and stage of disease.

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Differential effects of interleukin-4 in peptide induced autoimmunity

Abstract

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Keywords

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