Differential contribution of cis and trans gene transcription regulatory mechanisms in amygdala and prefrontal cortex and modulation by social stress

Eli Reuveni, Dmitry Getselter, Oded Oron, Evan Elliott

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

While both individual transcription factors and cis-acting sites have been studied in relation to psychiatric disorders, there is little knowledge of the relative contribution of trans-acting and cis-acting factors to gene transcription in the brain. Using an RNA-seq approach in mice bred from two evolutionary-distinct mice strains, we determined the contribution of cis and trans factors to gene expression in the prefrontal cortex and amygdala, two regions of the brain relevant to the stress response, and the contribution of cis and trans factors in the prefrontal cortex after Chronic Social Defeat (CSD) in mice. More genes were regulated by cis-regulatory factors in both brain regions, underlying the importance of cis-acting gene regulation in the brain. However, there was an increase in genes regulated by trans-regulatory mechanisms in the amygdala, compared to the prefrontal cortex. These genes were involved in synaptic functions, and were enriched for binding sites for transcription factors, including Egr1. CSD induced an increase in genes regulated by trans-regulatory mechanisms in the prefrontal cortex, and induced a pattern similar to the unstressed amygdala. Overall, we show brain site-specific patterns in cis and trans regulatory mechanisms, and show that these patterns can be modified by a psychological trigger.

Original languageEnglish
Article number6339
JournalScientific Reports
Volume8
Issue number1
DOIs
StatePublished - 20 Apr 2018

Bibliographical note

Publisher Copyright:
© 2018 The Author(s).

Funding

We would like to thank Dr. Marcela Karpuj and the staff of genomics unit at the Bar Ilan University Faculty of Medicine for their intellectual contribution to this manuscript. We would like to thank the Barts and London Genome Centre for their assistance in the sequencing analysis used in our validation studies. In addition, we would like to thank Sharon Manashirov for his assistance in producing Fig. 1 in this manuscript. This work was supported by the German Israeli Foundation grant 2293-2265 (to E.E) and Israel Science Foundation grant 1047/12 (to E.E).

FundersFunder number
German Israeli Foundation2293-2265
Israel Science Foundation1047/12

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