Differential aspects in ratio measurements of [Ca²⁺]_i relaxation in cardiomyocyte contraction following various drug treatments

This study is concerned with the analysis of the time dependency of[Ca²⁺]_i, monitored by indo-1-AM,via the ratiometric time response curve R(t) as measured during contractions of spontaneous or electrical stimulated cardiomyocytes (in culture). A mathematical formulation which describes the relaxation phase of R(t) was developed. By fitting formulation to the measured data of R(t), the extraction of characteristic parameters is feasible, which may reflect the factors regulating intracellular Ca concentration. The usefulness of the suggested formulation was examined by monitoring changes induced in those parameters following the exposure of the myocytes to different drugs, among which are: caffeine, ryanodine, thapsigargin db, cyclic AMP, isoprenaline, doxorubicin, and CI-IB-MECA.