Developmentally Regulated Novel Non-coding Anti-sense Regulators of mRNA Translation in Trypanosoma brucei

K. Shanmugha Rajan; Tirza Doniger; Smadar Cohen-Chalamish; Praveenkumar Rengaraj; Beathrice Galili; Saurav Aryal; Ron Unger; Christian Tschudi; Shulamit Michaeli

doi:10.1016/j.isci.2020.101780

Developmentally Regulated Novel Non-coding Anti-sense Regulators of mRNA Translation in Trypanosoma brucei

K. Shanmugha Rajan, Tirza Doniger, Smadar Cohen-Chalamish, Praveenkumar Rengaraj, Beathrice Galili, Saurav Aryal, Ron Unger, Christian Tschudi, Shulamit Michaeli

The Mina and Everard Goodman Faculty of Life Sciences - at Bar-Ilan University

Yale University

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The parasite Trypanosoma brucei is the causative agent of sleeping sickness and cycles between insect and mammalian hosts. The parasite appears to lack conventional transcriptional regulation of protein coding genes, and mRNAs are processed from polycistronic transcripts by the concerted action of trans-splicing and polyadenylation. Regulation of mRNA function is mediated mainly by RNA binding proteins affecting mRNA stability and translation. In this study, we describe the identification of 62 non-coding (nc) RNAs that are developmentally regulated and/or respond to stress. We characterized two novel anti-sense RNA regulators (TBsRNA-33 and 37) that originate from the rRNA loci, associate with ribosomes and polyribosomes, and interact in vivo with distinct mRNA species to regulate translation. Thus, this study suggests for the first-time anti-sense RNA regulators as an additional layer for controlling gene expression in these parasites.

Original language	English
Article number	101780
Journal	iScience
Volume	23
Issue number	12
DOIs	https://doi.org/10.1016/j.isci.2020.101780
State	Published - 18 Dec 2020

Bibliographical note

Publisher Copyright:
© 2020 The Authors

Funding

This work was supported by a grant from the Israel-US Binational Science Foundation (BSF), and NIH grant R01 AI 056333 to C.T. S.M. holds the David and Inez Myers Chair in RNA silencing of diseases.

Funders	Funder number
Israel–US Binational Science Foundation
National Institutes of Health	R01 AI 056333
United States - Israel Binational Science Foundation

Keywords

Molecular Biology
Omics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.isci.2020.101780

Cite this

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title = "Developmentally Regulated Novel Non-coding Anti-sense Regulators of mRNA Translation in Trypanosoma brucei",

abstract = "The parasite Trypanosoma brucei is the causative agent of sleeping sickness and cycles between insect and mammalian hosts. The parasite appears to lack conventional transcriptional regulation of protein coding genes, and mRNAs are processed from polycistronic transcripts by the concerted action of trans-splicing and polyadenylation. Regulation of mRNA function is mediated mainly by RNA binding proteins affecting mRNA stability and translation. In this study, we describe the identification of 62 non-coding (nc) RNAs that are developmentally regulated and/or respond to stress. We characterized two novel anti-sense RNA regulators (TBsRNA-33 and 37) that originate from the rRNA loci, associate with ribosomes and polyribosomes, and interact in vivo with distinct mRNA species to regulate translation. Thus, this study suggests for the first-time anti-sense RNA regulators as an additional layer for controlling gene expression in these parasites.",

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AU - Rajan, K. Shanmugha

AU - Doniger, Tirza

AU - Cohen-Chalamish, Smadar

AU - Rengaraj, Praveenkumar

AU - Galili, Beathrice

AU - Aryal, Saurav

AU - Unger, Ron

AU - Tschudi, Christian

AU - Michaeli, Shulamit

PY - 2020/12/18

Y1 - 2020/12/18

N2 - The parasite Trypanosoma brucei is the causative agent of sleeping sickness and cycles between insect and mammalian hosts. The parasite appears to lack conventional transcriptional regulation of protein coding genes, and mRNAs are processed from polycistronic transcripts by the concerted action of trans-splicing and polyadenylation. Regulation of mRNA function is mediated mainly by RNA binding proteins affecting mRNA stability and translation. In this study, we describe the identification of 62 non-coding (nc) RNAs that are developmentally regulated and/or respond to stress. We characterized two novel anti-sense RNA regulators (TBsRNA-33 and 37) that originate from the rRNA loci, associate with ribosomes and polyribosomes, and interact in vivo with distinct mRNA species to regulate translation. Thus, this study suggests for the first-time anti-sense RNA regulators as an additional layer for controlling gene expression in these parasites.

AB - The parasite Trypanosoma brucei is the causative agent of sleeping sickness and cycles between insect and mammalian hosts. The parasite appears to lack conventional transcriptional regulation of protein coding genes, and mRNAs are processed from polycistronic transcripts by the concerted action of trans-splicing and polyadenylation. Regulation of mRNA function is mediated mainly by RNA binding proteins affecting mRNA stability and translation. In this study, we describe the identification of 62 non-coding (nc) RNAs that are developmentally regulated and/or respond to stress. We characterized two novel anti-sense RNA regulators (TBsRNA-33 and 37) that originate from the rRNA loci, associate with ribosomes and polyribosomes, and interact in vivo with distinct mRNA species to regulate translation. Thus, this study suggests for the first-time anti-sense RNA regulators as an additional layer for controlling gene expression in these parasites.

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Developmentally Regulated Novel Non-coding Anti-sense Regulators of mRNA Translation in Trypanosoma brucei

Abstract

Bibliographical note

Funding

Keywords

UN SDGs

Access to Document

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