Development of Powdery Mildew Resistance in Cucumber Using CRISPR/Cas9-Mediated Mutagenesis of CsaMLO8

Yulia Shnaider, Elad Yigal, Dalia Rav-David, Ekaterina Pashkovsky, Diana Leibman, Michael Kravchik, Meital Shtarkman-Cohen, Amit Gal-On, Ziv Spiegelman

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Powdery mildew (PM) diseases may severely limit the production of various crops, including members of the family Cucurbitaceae. Successful PM infection relies on the Mildew Resistance Locus O (MLO) plant gene family, which encodes susceptibility factors essential for fungus penetration into the host cell. In cucumber (Cucumis sativus), natural mutations in CsaMLO8 confer resistance to the PM pathogen Podosphaera xanthii. Here, we used CRISPR/Cas9-mediated mutagenesis to generate PM resistance in the susceptible cucumber cultivar Ilan. Two transgene-free Csamlo8 CRISPR mutant lines (Csamlo-cr-1 and Csamlo-cr-2) were isolated, the first with a 5-bp deletion in exon 1, and the second harboring a 1,280-bp deletion and 10-bp insertion between exons 1 and 5. Both lines showed high resistance to PM under semicommercial growth conditions in the summer growing seasons of 2019 and 2021. These results provide the basis for generating transgene-free powdery mildew resistance in cucumber in any genetic background. This method can directly be employed on commercial cultivars and hybrid parental lines, and thereby substantially shorten and simplify the breeding process for PM resistance in cucumber.

Original languageEnglish
Pages (from-to)786-790
Number of pages5
Issue number5
StatePublished - May 2023
Externally publishedYes

Bibliographical note

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© 2023 American Phytopathological Society. All rights reserved.


  • CRISPR/Cas9
  • Cucumis sativus
  • MLO
  • Podosphaera xanthii
  • cucumber
  • powdery mildew
  • susceptibility gene


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