The healthcare industry is moving towards personalized medicine and patients seek greater role in their care. In this paper we describe a proof of concept of versatile optical sensor that potentially can be used for in vivo detection of biological events. The developed sensor comprises of a silver thin film glass slide and gold nanoparticles (GNPs) and allows detection of chemical changes using surface plasmon resonance (SPR), local surface plasmon resonance (LSPR) and fluorescence lifetime detection modalities. A change in spectrum was observed after removal of GNPs by 2-mercaptoethanol. Moreover, GNP conjugated to fluorescein were tested using fluorescence lifetime microscopy (FLIM) to enhance sensing capabilities and provide additional dimension for detection. The sensor has the potential to provide real-time analyses of different biological molecules, such as antibody and antigen, receptor and ligand, and complementary DNA fragments.
|Title of host publication||Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVI|
|Editors||Ewa M. Goldys, Dror Fixler, Dan V. Nicolau|
|State||Published - 2019|
|Event||Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVI 2019 - San Francisco, United States|
Duration: 3 Feb 2019 → 4 Feb 2019
|Name||Progress in Biomedical Optics and Imaging - Proceedings of SPIE|
|Conference||Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVI 2019|
|Period||3/02/19 → 4/02/19|
Bibliographical noteFunding Information:
The work described in this proceeding is supported by the Israeli Ministry of Science, Technology, and
© 2019 SPIE.
- Fluorescence lifetime imaging
- Gold nanoparticles
- Local Surface Plasmon Resonance (LSPR)