Development of colonies in vitro of mitogen-stimulated mouse T lymphocytes

B. Sredni; Y. Kalechman; H. Michlin; L. A. Rozenszajn

doi:10.1038/259130a0

Development of colonies in vitro of mitogen-stimulated mouse T lymphocytes

B. Sredni, Y. Kalechman, H. Michlin, L. A. Rozenszajn

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Abstract

TECHNIQUES have been developed for the growth of haemopoietic cells (see refs 1 and 2), human T lymphocytes³ and mouse B lymphocytes ⁴ on soft agar. Experiments with mouse lymphocytes suggest that concanavalin A (con A) and phytohaemagglutinin (PHA) stimulate T cells ^5,6, whereas pokeweed induces proliferation of both B and T cells⁷. Based on previously described techniques for obtaining clonal growth of human lymphocyte colonies in vitro³, a procedure has now been developed for inducing clonal proliferation of mitogen-stimulated mouse lymphocytes using a two-layer soft agar technique.

Original language	English
Pages (from-to)	130-132
Number of pages	3
Journal	Nature
Volume	259
Issue number	5539
DOIs	https://doi.org/10.1038/259130a0
State	Published - 1976

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author = "B. Sredni and Y. Kalechman and H. Michlin and Rozenszajn, {L. A.}",

year = "1976",

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AU - Kalechman, Y.

AU - Michlin, H.

AU - Rozenszajn, L. A.

PY - 1976

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Development of colonies in vitro of mitogen-stimulated mouse T lymphocytes

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