Development of colonies in vitro of mitogen-stimulated mouse T lymphocytes

B. Sredni, Y. Kalechman, H. Michlin, L. A. Rozenszajn

Research output: Contribution to journalArticlepeer-review

35 Scopus citations


TECHNIQUES have been developed for the growth of haemopoietic cells (see refs 1 and 2), human T lymphocytes3 and mouse B lymphocytes 4 on soft agar. Experiments with mouse lymphocytes suggest that concanavalin A (con A) and phytohaemagglutinin (PHA) stimulate T cells 5,6, whereas pokeweed induces proliferation of both B and T cells7. Based on previously described techniques for obtaining clonal growth of human lymphocyte colonies in vitro3, a procedure has now been developed for inducing clonal proliferation of mitogen-stimulated mouse lymphocytes using a two-layer soft agar technique.

Original languageEnglish
Pages (from-to)130-132
Number of pages3
Issue number5539
StatePublished - 1976


Dive into the research topics of 'Development of colonies in vitro of mitogen-stimulated mouse T lymphocytes'. Together they form a unique fingerprint.

Cite this