Development of a novel Clq immunoadsorbent for removal of circulating immunecomplexes: Quantitative isolation of hepatitis B virus surface antigen and immunecomplexes

Y. Gazitt; S. Margel; A. Lerner; J. R. Wands; D. Shouval

doi:10.1016/0165-2478(85)90135-x

Development of a novel Clq immunoadsorbent for removal of circulating immunecomplexes: Quantitative isolation of hepatitis B virus surface antigen and immunecomplexes

Y. Gazitt, S. Margel, A. Lerner, J. R. Wands, D. Shouval

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

A technique for large scale production of human C1q from plasma by affinity chromatography on an anti-C1q column is described. Affinity purified C1q was covalently coupled to a newly developed agarose polyacrolein microsphere beads immunoadsorbent. This immunoadsorbent was utilized for quantitative removal of artificially formed bovine serum albumin (BSA)-anti-BSA immune complexes (IC). The C1q affinity column was then used for the isolation of immunecomplexes containing hepatitis B virus (HBV) surface antigen (HBsAg) from serum of an HBsAg carrier. Identical columns may be utilized for quantitative removal of a variety of IC from blood of patients with infectious and autoimmune diseases, as well as neoplastic diseases. Furthermore, dissociated immunecomplexes will provide an additional source for purification of specific antigens.

Original language	English
Pages (from-to)	1-8
Number of pages	8
Journal	Immunology Letters
Volume	11
Issue number	1
DOIs	https://doi.org/10.1016/0165-2478(85)90135-x
State	Published - 1985
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by a grant from Galil Advanced Technologies, Israel (Y.G.). We would like to thank Mrs. G. Adarei and Mrs. R. Adler for their excellent technical assistance. We also would like to thank Dr. N. Mani from the Hadassah blood bank for supplying the outdated blood.

Funding

This work was supported by a grant from Galil Advanced Technologies, Israel (Y.G.). We would like to thank Mrs. G. Adarei and Mrs. R. Adler for their excellent technical assistance. We also would like to thank Dr. N. Mani from the Hadassah blood bank for supplying the outdated blood.

Funders	Funder number
Galil Advanced Technologies, Israel

Keywords

HBsAg
affinity purification
immune complex C1q

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0165-2478(85)90135-x

Cite this

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title = "Development of a novel Clq immunoadsorbent for removal of circulating immunecomplexes: Quantitative isolation of hepatitis B virus surface antigen and immunecomplexes",

abstract = "A technique for large scale production of human C1q from plasma by affinity chromatography on an anti-C1q column is described. Affinity purified C1q was covalently coupled to a newly developed agarose polyacrolein microsphere beads immunoadsorbent. This immunoadsorbent was utilized for quantitative removal of artificially formed bovine serum albumin (BSA)-anti-BSA immune complexes (IC). The C1q affinity column was then used for the isolation of immunecomplexes containing hepatitis B virus (HBV) surface antigen (HBsAg) from serum of an HBsAg carrier. Identical columns may be utilized for quantitative removal of a variety of IC from blood of patients with infectious and autoimmune diseases, as well as neoplastic diseases. Furthermore, dissociated immunecomplexes will provide an additional source for purification of specific antigens.",

keywords = "HBsAg, affinity purification, immune complex C1q",

author = "Y. Gazitt and S. Margel and A. Lerner and Wands, {J. R.} and D. Shouval",

note = "Funding Information: This work was supported by a grant from Galil Advanced Technologies, Israel (Y.G.). We would like to thank Mrs. G. Adarei and Mrs. R. Adler for their excellent technical assistance. We also would like to thank Dr. N. Mani from the Hadassah blood bank for supplying the outdated blood.",

year = "1985",

doi = "10.1016/0165-2478(85)90135-x",

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AU - Gazitt, Y.

AU - Margel, S.

AU - Lerner, A.

AU - Wands, J. R.

AU - Shouval, D.

N1 - Funding Information: This work was supported by a grant from Galil Advanced Technologies, Israel (Y.G.). We would like to thank Mrs. G. Adarei and Mrs. R. Adler for their excellent technical assistance. We also would like to thank Dr. N. Mani from the Hadassah blood bank for supplying the outdated blood.

PY - 1985

Y1 - 1985

N2 - A technique for large scale production of human C1q from plasma by affinity chromatography on an anti-C1q column is described. Affinity purified C1q was covalently coupled to a newly developed agarose polyacrolein microsphere beads immunoadsorbent. This immunoadsorbent was utilized for quantitative removal of artificially formed bovine serum albumin (BSA)-anti-BSA immune complexes (IC). The C1q affinity column was then used for the isolation of immunecomplexes containing hepatitis B virus (HBV) surface antigen (HBsAg) from serum of an HBsAg carrier. Identical columns may be utilized for quantitative removal of a variety of IC from blood of patients with infectious and autoimmune diseases, as well as neoplastic diseases. Furthermore, dissociated immunecomplexes will provide an additional source for purification of specific antigens.

AB - A technique for large scale production of human C1q from plasma by affinity chromatography on an anti-C1q column is described. Affinity purified C1q was covalently coupled to a newly developed agarose polyacrolein microsphere beads immunoadsorbent. This immunoadsorbent was utilized for quantitative removal of artificially formed bovine serum albumin (BSA)-anti-BSA immune complexes (IC). The C1q affinity column was then used for the isolation of immunecomplexes containing hepatitis B virus (HBV) surface antigen (HBsAg) from serum of an HBsAg carrier. Identical columns may be utilized for quantitative removal of a variety of IC from blood of patients with infectious and autoimmune diseases, as well as neoplastic diseases. Furthermore, dissociated immunecomplexes will provide an additional source for purification of specific antigens.

KW - HBsAg

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Development of a novel Clq immunoadsorbent for removal of circulating immunecomplexes: Quantitative isolation of hepatitis B virus surface antigen and immunecomplexes

Abstract

Bibliographical note

Funding

Keywords

UN SDGs

Access to Document

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