Determining epithelial contribution to in vivo mesenchymal tumour expression signature using species-specific microarray profiling analysis of xenografts

E. Purdom, C. Restall, R. A. Busuttil, H. Schluter, A. Boussioutas, E. W. Thompson, R. L. Anderson, T. P. Speed, I. Haviv

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Gene expression profiling using microarrays and xenograft transplants of human cancer cell lines are both popular tools to investigate human cancer. However, the undefined degree of cross hybridization between the mouse and human genomes hinders the use of microarrays to characterize gene expression of both the host and the cancer cell within the xenograft. Since an increasingly recognized aspect of cancer is the host response (or cancer-stroma interaction), we describe here a bioinformatic manipulation of the Affymetrix profiling that allows interrogation of the gene expression of both the mouse host and the human tumour. Evidence of microenvironmental regulation of epithelial mesenchymal transition of the tumour component in vivo is resolved against a background of mesenchymal gene expression. This tool could allow deeper insight to the mechanism of action of anti-cancer drugs, as typically novel drug efficacy is being tested in xenograft systems.

Original languageEnglish
Pages (from-to)14-29
Number of pages16
JournalGenetics Research
Volume95
Issue number1
DOIs
StatePublished - Feb 2013

Fingerprint

Dive into the research topics of 'Determining epithelial contribution to in vivo mesenchymal tumour expression signature using species-specific microarray profiling analysis of xenografts'. Together they form a unique fingerprint.

Cite this