TY - JOUR
T1 - Detection of waterborne and airborne formaldehyde
T2 - From amperometric chemosensing to a visual biosensor based on alcohol oxidase
AU - Sigawi, Sasi
AU - Smutok, Oleh
AU - Demkiv, Olha
AU - Gayda, Galina
AU - Vus, Bohdan
AU - Nitzan, Yeshayahu
AU - Gonchar, Mykhailo
AU - Nisnevitch, Marina
PY - 2014/2/11
Y1 - 2014/2/11
N2 - A laboratory prototype of a microcomputer-based analyzer was developed for quantitative determination of formaldehyde in liquid samples, based on catalytic chemosensing elements. It was shown that selectivity for the target analyte could be increased by modulating the working electrode potential. Analytical parameters of three variants of the amperometric analyzer that differed in the chemical structure/configuration of the working electrode were studied. The constructed analyzer was tested on wastewater solutions that contained formaldehyde. A simple low-cost biosensor was developed for semi-quantitative detection of airborne formaldehyde in concentrations exceeding the threshold level. This biosensor is based on a change in the color of a solution that contains a mixture of alcohol oxidase from the yeast Hansenula polymorpha, horseradish peroxidase and a chromogen, following exposure to airborne formaldehyde. The solution is enclosed within a membrane device, which is permeable to formaldehyde vapors. The most efficient and sensitive biosensor for detecting formaldehyde was the one that contained alcohol oxidase with an activity of 1.2 U.mL-1. The biosensor requires no special instrumentation and enables rapid visual detection of airborne formaldehyde at concentrations, which are hazardous to human health.
AB - A laboratory prototype of a microcomputer-based analyzer was developed for quantitative determination of formaldehyde in liquid samples, based on catalytic chemosensing elements. It was shown that selectivity for the target analyte could be increased by modulating the working electrode potential. Analytical parameters of three variants of the amperometric analyzer that differed in the chemical structure/configuration of the working electrode were studied. The constructed analyzer was tested on wastewater solutions that contained formaldehyde. A simple low-cost biosensor was developed for semi-quantitative detection of airborne formaldehyde in concentrations exceeding the threshold level. This biosensor is based on a change in the color of a solution that contains a mixture of alcohol oxidase from the yeast Hansenula polymorpha, horseradish peroxidase and a chromogen, following exposure to airborne formaldehyde. The solution is enclosed within a membrane device, which is permeable to formaldehyde vapors. The most efficient and sensitive biosensor for detecting formaldehyde was the one that contained alcohol oxidase with an activity of 1.2 U.mL-1. The biosensor requires no special instrumentation and enables rapid visual detection of airborne formaldehyde at concentrations, which are hazardous to human health.
KW - Biosensor, alcohol oxidase
KW - Chemosensing electrode
KW - Formaldehyde
KW - Hansenula polymorpha
KW - Microcomputer-based analyzer
UR - http://www.scopus.com/inward/record.url?scp=84894834070&partnerID=8YFLogxK
U2 - 10.3390/ma7021055
DO - 10.3390/ma7021055
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AN - SCOPUS:84894834070
SN - 1996-1944
VL - 7
SP - 1055
EP - 1068
JO - Materials
JF - Materials
IS - 2
ER -
