Detection of subclinical peste des petits ruminants virus infection in experimental cattle

A. Sen; P. Saravanan; V. Balamurugan; V. Bhanuprakash; G. Venkatesan; J. Sarkar; K. K. Rajak; A. Ahuja; V. Yadav; S. B. Sudhakar; S. Parida; R. K. Singh

doi:10.1007/s13337-014-0213-0

Detection of subclinical peste des petits ruminants virus infection in experimental cattle

A. Sen, P. Saravanan, V. Balamurugan, V. Bhanuprakash, G. Venkatesan, J. Sarkar, K. K. Rajak, A. Ahuja, V. Yadav, S. B. Sudhakar, S. Parida, R. K. Singh

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

The present study was undertaken to investigate the possible involvement of cattle in the epidemiology of peste des petits ruminants (PPR) as subclinical carriers. Cattle were exposed experimentally to PPR virus (PPRV) infection or placed in contact with PPR infected goats. Clinical samples including heparinized/EDTA blood, plasma, peripheral blood monocyte cells (PBMCs) and clotted blood (for serum) were collected periodically from 21 days post infection (dpi) to 397 dpi (21, 45, 50, 57, 65, 95, 111, 119, 148, 190, 203 and 397 dpi) and tested for PPRV antigen, nucleic acid and antibody. Exposed cattle seroconverted and maintained PPRV specific haemagglutinin antibodies and detectable PPRV antigen/nucleic acid in blood, plasma and PBMCs from 21 to 397 dpi. PPRV was recovered from blood and PBMC collected from experimental animals at 21 dpi, initially in B95a cells and then adapted to Vero cells. The study indicated that PPRV can infect cattle subclinically and PPRV antigen/nucleic acid persist in cattle for at least 397 days.

Original language	English
Pages (from-to)	408-411
Number of pages	4
Journal	VirusDisease
Volume	25
Issue number	3
DOIs	https://doi.org/10.1007/s13337-014-0213-0
State	Published - 9 Sep 2014
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2014, Indian Virological Society.

Funding

Funders	Funder number
Biotechnology and Biological Sciences Research Council	BB/J020478/1, BB/L004801/1

Keywords

Cattle
Experimental studies
PPR
Subclinical infection

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s13337-014-0213-0

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abstract = "The present study was undertaken to investigate the possible involvement of cattle in the epidemiology of peste des petits ruminants (PPR) as subclinical carriers. Cattle were exposed experimentally to PPR virus (PPRV) infection or placed in contact with PPR infected goats. Clinical samples including heparinized/EDTA blood, plasma, peripheral blood monocyte cells (PBMCs) and clotted blood (for serum) were collected periodically from 21 days post infection (dpi) to 397 dpi (21, 45, 50, 57, 65, 95, 111, 119, 148, 190, 203 and 397 dpi) and tested for PPRV antigen, nucleic acid and antibody. Exposed cattle seroconverted and maintained PPRV specific haemagglutinin antibodies and detectable PPRV antigen/nucleic acid in blood, plasma and PBMCs from 21 to 397 dpi. PPRV was recovered from blood and PBMC collected from experimental animals at 21 dpi, initially in B95a cells and then adapted to Vero cells. The study indicated that PPRV can infect cattle subclinically and PPRV antigen/nucleic acid persist in cattle for at least 397 days.",

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AU - Venkatesan, G.

AU - Sarkar, J.

AU - Rajak, K. K.

AU - Ahuja, A.

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AU - Parida, S.

AU - Singh, R. K.

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Detection of subclinical peste des petits ruminants virus infection in experimental cattle

Abstract

Bibliographical note

Funding

Keywords

UN SDGs

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