Detection of soluble integrin in human serum

Ilan Bank, Peretz Weiss, Ram Doolman, Mazal Book, Ben Ami Sela

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

An enzyme-linked immunosorbent assay (ELISA) for the detection and quantitation of soluble α1β1 integrins (sα1) in human serum samples was developed. Solid phase-bound anti-α1 integrin monoclonal antibody (mAb) TS2/7 was used to capture sα1, and mAb 1B3.1 was used to detect the immobilized integrin. An extract of human placenta (PE) containing 340 ng/mL of VLA-1 molecules served as a positive control, and serum samples from normal donors and patients were assayed. Optimal binding of anti-α1 integrin mAb 1B3.1, expressed as specific optical density (OD), was obtained when a 5 μg/mL solution of anti-α1 integrin 'capture' mAb TS2/7 was immobilized to the wells and the PE was added. Solutions of albumin or collagen, in contrast, did not result in binding, confirming the specificity of the assay for sα1. Furthermore, the specific OD of the wells correlated directly with the concentration of PE. A concentration of sα1 above that of a 1:100 dilution of PE-that is, >3.4 ng/mL of integrin, in which the intra-assay correlation of variance was <5.7%, was found in 5 of 8, 3 of 8, and 6 of 9 serum samples from normal individuals, patients with connective tissue diseases (CTD), and patients with liver diseases (LD), respectively. These results suggest, for the first time, that sα1 are present in healthy and diseased human serum.

Original languageEnglish
Pages (from-to)599-604
Number of pages6
JournalJournal of Laboratory and Clinical Medicine
Volume134
Issue number6
DOIs
StatePublished - Dec 1999

Bibliographical note

Funding Information:
Supported by the Tel Aviv University Research Authority.

Funding

Supported by the Tel Aviv University Research Authority.

FundersFunder number
Tel Aviv University

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