Detection of new delhi metallo-beta-lactamase and extended-spectrum beta-lactamase genes in escherichia coli isolated from mastitic milk samples

S. Ghatak; A. Singha; A. Sen; C. Guha; A. Ahuja; U. Bhattacharjee; S. Das; N. R. Pradhan; K. Puro; C. Jana; T. K. Dey; K. L. Prashantkumar; A. Das; I. Shakuntala; U. Biswas; P. S. Jana

doi:10.1111/tbed.12119

Detection of new delhi metallo-beta-lactamase and extended-spectrum beta-lactamase genes in escherichia coli isolated from mastitic milk samples

S. Ghatak, A. Singha, A. Sen, C. Guha, A. Ahuja, U. Bhattacharjee, S. Das, N. R. Pradhan, K. Puro, C. Jana, T. K. Dey, K. L. Prashantkumar, A. Das, I. Shakuntala, U. Biswas, P. S. Jana

Research output: Contribution to journal › Article › peer-review

63 Scopus citations

Abstract

Summary: In this study, eight Escherichia coli isolates were obtained from milk samples of dairy cattle suffering from clinical/subclinical mastitis. Isolates were characterized for antimicrobial resistance traits and virulence genes. Results revealed that one isolate was harbouring New Delhi metallo-beta-lactamase gene (bla_NDM). Cloning and sequencing of the PCR amplicon confirmed the identity of the gene (GenBank accession no. KC769583) having 100% homology with bla_NDM-5 (GenBank accession no. JN104597.1), and this isolate was susceptible to colistin, chloramphenicol and tetracycline only. Moreover, another isolate carried extended-spectrum beta-lactamase (ESBL) gene - bla_CTX-M, and all isolates possessed bla_TEM gene. Of the eight isolates, only one isolate was positive for shiga toxin gene (stx2), and none were harbouring stx1 gene. Occurrence of New Delhi metallo-beta-lactamase (bla_NDM) in one E. coli isolate and ESBL genes in other isolates poses a potential threat to human health following possible entry and spread through food chain.

Original language	English
Pages (from-to)	385-389
Number of pages	5
Journal	Transboundary and Emerging Diseases
Volume	60
Issue number	5
DOIs	https://doi.org/10.1111/tbed.12119
State	Published - Oct 2013
Externally published	Yes

Keywords

Escherichia coli
Extended-spectrum beta-lactamase
Metallo-beta-lactamase
Milk

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1111/tbed.12119

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Ghatak, S., Singha, A., Sen, A., Guha, C., Ahuja, A., Bhattacharjee, U., Das, S., Pradhan, N. R., Puro, K., Jana, C., Dey, T. K., Prashantkumar, K. L., Das, A., Shakuntala, I., Biswas, U., & Jana, P. S. (2013). Detection of new delhi metallo-beta-lactamase and extended-spectrum beta-lactamase genes in escherichia coli isolated from mastitic milk samples. Transboundary and Emerging Diseases, 60(5), 385-389. https://doi.org/10.1111/tbed.12119

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title = "Detection of new delhi metallo-beta-lactamase and extended-spectrum beta-lactamase genes in escherichia coli isolated from mastitic milk samples",

abstract = "Summary: In this study, eight Escherichia coli isolates were obtained from milk samples of dairy cattle suffering from clinical/subclinical mastitis. Isolates were characterized for antimicrobial resistance traits and virulence genes. Results revealed that one isolate was harbouring New Delhi metallo-beta-lactamase gene (blaNDM). Cloning and sequencing of the PCR amplicon confirmed the identity of the gene (GenBank accession no. KC769583) having 100% homology with blaNDM-5 (GenBank accession no. JN104597.1), and this isolate was susceptible to colistin, chloramphenicol and tetracycline only. Moreover, another isolate carried extended-spectrum beta-lactamase (ESBL) gene - blaCTX-M, and all isolates possessed blaTEM gene. Of the eight isolates, only one isolate was positive for shiga toxin gene (stx2), and none were harbouring stx1 gene. Occurrence of New Delhi metallo-beta-lactamase (blaNDM) in one E. coli isolate and ESBL genes in other isolates poses a potential threat to human health following possible entry and spread through food chain.",

keywords = "Escherichia coli, Extended-spectrum beta-lactamase, Metallo-beta-lactamase, Milk",

author = "S. Ghatak and A. Singha and A. Sen and C. Guha and A. Ahuja and U. Bhattacharjee and S. Das and Pradhan, {N. R.} and K. Puro and C. Jana and Dey, {T. K.} and Prashantkumar, {K. L.} and A. Das and I. Shakuntala and U. Biswas and Jana, {P. S.}",

year = "2013",

month = oct,

doi = "10.1111/tbed.12119",

language = "אנגלית",

volume = "60",

pages = "385--389",

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Ghatak, S, Singha, A, Sen, A, Guha, C, Ahuja, A, Bhattacharjee, U, Das, S, Pradhan, NR, Puro, K, Jana, C, Dey, TK, Prashantkumar, KL, Das, A, Shakuntala, I, Biswas, U & Jana, PS 2013, 'Detection of new delhi metallo-beta-lactamase and extended-spectrum beta-lactamase genes in escherichia coli isolated from mastitic milk samples', Transboundary and Emerging Diseases, vol. 60, no. 5, pp. 385-389. https://doi.org/10.1111/tbed.12119

TY - JOUR

T1 - Detection of new delhi metallo-beta-lactamase and extended-spectrum beta-lactamase genes in escherichia coli isolated from mastitic milk samples

AU - Ghatak, S.

AU - Singha, A.

AU - Sen, A.

AU - Guha, C.

AU - Ahuja, A.

AU - Bhattacharjee, U.

AU - Das, S.

AU - Pradhan, N. R.

AU - Puro, K.

AU - Jana, C.

AU - Dey, T. K.

AU - Prashantkumar, K. L.

AU - Das, A.

AU - Shakuntala, I.

AU - Biswas, U.

AU - Jana, P. S.

PY - 2013/10

Y1 - 2013/10

N2 - Summary: In this study, eight Escherichia coli isolates were obtained from milk samples of dairy cattle suffering from clinical/subclinical mastitis. Isolates were characterized for antimicrobial resistance traits and virulence genes. Results revealed that one isolate was harbouring New Delhi metallo-beta-lactamase gene (blaNDM). Cloning and sequencing of the PCR amplicon confirmed the identity of the gene (GenBank accession no. KC769583) having 100% homology with blaNDM-5 (GenBank accession no. JN104597.1), and this isolate was susceptible to colistin, chloramphenicol and tetracycline only. Moreover, another isolate carried extended-spectrum beta-lactamase (ESBL) gene - blaCTX-M, and all isolates possessed blaTEM gene. Of the eight isolates, only one isolate was positive for shiga toxin gene (stx2), and none were harbouring stx1 gene. Occurrence of New Delhi metallo-beta-lactamase (blaNDM) in one E. coli isolate and ESBL genes in other isolates poses a potential threat to human health following possible entry and spread through food chain.

AB - Summary: In this study, eight Escherichia coli isolates were obtained from milk samples of dairy cattle suffering from clinical/subclinical mastitis. Isolates were characterized for antimicrobial resistance traits and virulence genes. Results revealed that one isolate was harbouring New Delhi metallo-beta-lactamase gene (blaNDM). Cloning and sequencing of the PCR amplicon confirmed the identity of the gene (GenBank accession no. KC769583) having 100% homology with blaNDM-5 (GenBank accession no. JN104597.1), and this isolate was susceptible to colistin, chloramphenicol and tetracycline only. Moreover, another isolate carried extended-spectrum beta-lactamase (ESBL) gene - blaCTX-M, and all isolates possessed blaTEM gene. Of the eight isolates, only one isolate was positive for shiga toxin gene (stx2), and none were harbouring stx1 gene. Occurrence of New Delhi metallo-beta-lactamase (blaNDM) in one E. coli isolate and ESBL genes in other isolates poses a potential threat to human health following possible entry and spread through food chain.

KW - Escherichia coli

KW - Extended-spectrum beta-lactamase

KW - Metallo-beta-lactamase

KW - Milk

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U2 - 10.1111/tbed.12119

DO - 10.1111/tbed.12119

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C2 - 23870003

AN - SCOPUS:84883655260

SN - 1865-1674

VL - 60

SP - 385

EP - 389

JO - Transboundary and Emerging Diseases

JF - Transboundary and Emerging Diseases

IS - 5

ER -

Detection of new delhi metallo-beta-lactamase and extended-spectrum beta-lactamase genes in escherichia coli isolated from mastitic milk samples

Abstract

Keywords

UN SDGs

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