Abstract
A sensitive and rapid method for detecting fluorescent dyes at low concentrations in homogenous solution is experimentally demonstrated. Fluorescent-labeled DNA probes are detected by attaching magnetic beads and applying alternating magnetic field gradient. This condenses the fluorescent probes into a small detection volume and eliminates the scattering noise from solution by synchronous detection. For DNA probes concentration of 1·10-13 M the detection signal was 3.3 times higher than the noise, thereby implying detection sensitivity of 3·10-14 M.
| Original language | English |
|---|---|
| Pages (from-to) | 19253-19259 |
| Number of pages | 7 |
| Journal | Optics Express |
| Volume | 16 |
| Issue number | 23 |
| DOIs | |
| State | Published - 10 Nov 2008 |
| Externally published | Yes |
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