Detection of Extended-Spectrum β-Lactamase-Producing Escherichia coli Using Infrared Microscopy and Machine-Learning Algorithms

Uraib Sharaha, Eladio Rodriguez-Diaz, Orli Sagi, Klaris Riesenberg, Itshak Lapidot, Yoram Segal, Irving J. Bigio, Mahmoud Huleihel, Ahmad Salman

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


The spread of multidrug resistant bacteria has become a global concern. One of the most important and emergent classes of multidrug-resistant bacteria is extended-spectrum β-lactamase-producing bacteria (ESBL-positive = ESBL+). Due to widespread and continuous evolution of ESBL-producing bacteria, they become increasingly resistant to many of the commonly used antibiotics, leading to an increase in the mortality associated with resulting infections. Timely detection of ESBL-producing bacteria and rapid determination of their susceptibility to appropriate antibiotics can reduce the spread of these bacteria and the consequent complications. Routine methods used for the detection of ESBL-producing bacteria are time-consuming, requiring at least 48 h to obtain results. In this study, we evaluated the potential of infrared spectroscopic microscopy, combined with multivariate analysis for rapid detection of ESBL-producing Escherichia coli (E. coli) isolated from urinary-tract infection (UTI) samples. Our measurements were conducted on 837 samples of uropathogenic E. coli (UPEC), including 268 ESBL+ and 569 ESBL-negative (ESBL-) samples. All samples were obtained from bacterial colonies after 24 h culture (first culture) from midstream patients' urine. Our results revealed that it is possible to detect ESBL-producing bacteria, with a 97% success rate, 99% sensitivity, and 94% specificity for the tested samples, in a time span of few minutes following the first culture.

Original languageEnglish
Pages (from-to)2525-2530
Number of pages6
JournalAnalytical Chemistry
Issue number3
StatePublished - 5 Feb 2019
Externally publishedYes

Bibliographical note

Funding Information:
Financial support by SCE internal research funding is gratefully acknowledged.

Publisher Copyright:
© 2019 American Chemical Society.


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