Detection of antibodies specific for foot-and-mouth disease virus infection using indirect ELISA based on recombinant nonstructural protein 2B

Jitendra K. Biswal, Sarita Jena, Jajati K. Mohapatra, Punam Bisht, Bramhadev Pattnaik

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Foot-and-mouth disease (FMD) is a highly contagious viral disease of transboundary importance. In India, since the launch of the FMD control programme, there has been a substantial increase in the vaccinated bovine population. In this scenario, there is a need for additional locally developed non-structural protein (NSP)-based immnoassays for efficient identification of FMD virus (FMDV)-infected animals in the vaccinated population. The 2B NSP of FMDV, lacking the transmembrane domain (Δ2B), was expressed successfully in a prokaryotic system, and an indirect ELISA (I-ELISA) was developed and validated in this study. The diagnostic sensitivity and specificity of the Δ2B I-ELISA were found to be 95.3% and 94.6%, respectively. In experimentally infected cattle, the assay could consistently detect Δ2B-NSP-specific antibodies from 10 to approximately 400 days postinfection. The assay was further validated with bovine serum samples collected randomly from different parts of the country. The performance of the Δ2B I-ELISA was compared with the in-house r3AB3 I-ELISA, and the overall concordance in test results was found to be 86.49%. The Δ2B I-ELISA could be useful as a screening or confirmatory assay in the surveillance of FMD irrespective of vaccination.

Original languageEnglish
Pages (from-to)1641-1650
Number of pages10
JournalArchives of Virology
Volume159
Issue number7
DOIs
StatePublished - Jul 2014
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by Indian Council of Agricultural Research under the project IXX08487. The assistance of Mr. N. S. Singh in sorting the serum samples is appreciated.

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