Background: The α1β1 integrin is a cell surface membrane heterodimer composed of noncovalently linked !1 and "1 polypeptides that is up-regulated on activated and proliferating mesangial cells.Methods: A double-sandwich enzyme-linked immunosorbent assay that detects !1 integrin in a specific and dosedependent manner at concentrations greater than 150 ng/mL was used to evaluate whether intact !1 polypeptides are secreted in the urine samples of 29 patients with various kidney diseases and in those of 5 healthy individuals.Results: α1 Integrin was detected in 8 of the 29 patients including 3 of 3 patients with biopsy-proven immunoglobulin A nephropathy and 3 of 3 clinically suspected but non-biopsy-proven immunoglobulin A nephropathy with evidence of active nephritis. No !1 integrins were found in samples of 5 healthy controls.Conclusions: !1 Integrin polypeptides can be detected in human urine, particularly in immunoglobulin A nephropathy.Further extensive studies are required to clarify the significance of secretion of !1 integrins in urine ofpatients with kidney disease. Copyright @ 2008 American Federation for Medical Research.
|Number of pages||6|
|Journal||Journal of Investigative Medicine|
|State||Published - Mar 2008|
- IgA nephropathy α1 integrin