Design principles for noninvasive, longitudinal and quantitative cell tracking with nanoparticle-based CT imaging

Rinat Meir, Oshra Betzer, Menachem Motiei, Noam Kronfeld, Chaya Brodie, Rachela Popovtzer

Research output: Contribution to journalArticlepeer-review

56 Scopus citations

Abstract

Contradictory results in clinical trials are preventing the advancement and implementation of cell-based therapy. To explain such results, there is a need to uncover the mystery regarding the fate of the transplanted cells. To answer this need, we developed a technique for noninvasive in vivo cell tracking, which uses gold nanoparticles as contrast agents for CT imaging. Herein, we investigate the design principles of this technique for intramuscular transplantation of therapeutic cells. Longitudinal studies were performed, displaying the ability to track cells over long periods of time. As few as 500 cells could be detected and a way to quantify the number of cells visualized by CT was demonstrated. Moreover, monitoring of cell functionality was demonstrated on a mouse model of Duchenne muscular dystrophy. This cell-tracking technology has the potential to become an essential tool in pre-clinical as well as clinical trials and to advance the future of cell therapy.

Original languageEnglish
Pages (from-to)421-429
Number of pages9
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume13
Issue number2
DOIs
StatePublished - 1 Feb 2017

Bibliographical note

Publisher Copyright:
© 2016 Elsevier Inc.

Keywords

  • Cell therapy
  • Cell tracking
  • Computed tomography
  • Imaging
  • Nanoparticles

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